Products

    • Illumina DNA Prep product line

    Innovative library preparation

    Save time and gain lab efficiencies with fast, flexible sequencing library preparation

    Scientist holding reagent tube with dark liquid, NovaSeq 6000 blurry in background

    Illumina DNA Prep chemistry powers fast workflows

    Illumina DNA Prep products represent the latest revolution in Illumina library prep chemistry. Unique bead-linked transposome chemistry integrates DNA extraction, fragmentation, library preparation, and library normalization to deliver the fastest, most flexible whole-genome and targeted enrichment workflows in the Illumina library prep portfolio.

    Illumina DNA Prep products

    Each kit generates sequencing-ready libraries featuring consistent insert sizes, uniform coverage, and exceptional data quality, regardless of DNA input amount or type, for use in various applications.

    Illumina DNA Prep

    A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

    Illumina DNA Prep with Enrichment

    A complete targeted resequencing solution for a wide range of applications, with fast integrated workflow and options for custom and fixed enrichment panels.

    Illumina DNA PCR-Free Prep

    An efficient, fast, and integrated library preparation workflow that yields highly accurate data for sensitive sequencing applications.

    Key features and benefits

    Fast library prep

    Bead-linked transposome chemistry integrates multiple steps to deliver the fastest library preparation in the Illumina library prep portfolio. Prepare libraries in ~1.5–6.5 hours.

    Numerous applications

    Wide range of sequencing applications, including whole-genome sequencing of any species (large or small genomes), exome sequencing, and disease- or phenotype targeted sequencing with fixed, predesigned panels.

    Streamlined workflow

    User-friendly, automation-compatible workflow combines DNA fragmentation and adapter ligation and eliminates the need for library quantification.

    High data quality

    Bead-based chemistry minimizes bias and opportunities for error, resulting in highly reproducible high-quality sequencing data with even genome coverage.

    Multiple sample types

    Flexible protocol accommodates a broad range of sample input types, including blood, saliva, and formalin-fixed, paraffin-embedded (FFPE) tissue.

    Broad DNA input range

    Broad DNA input range of ~1–1000 ng. The required input varies based on complexity of genome being sequenced (eg, small vs large genomes) and kit (whole-genome vs enrichment).

    • Data sheet PDF < 1 MB 1 versions
    How Illumina DNA Prep technology works: (A) Bead-linked transposomes mediate the simultaneous fragmentation of gDNA and the addition of Illumina sequencing primers. (B) Reduced-cycle PCR amplification amplifies sequencing ready DNA fragments and adds indexes and adapters. (C) Sequencing-ready fragments are washed and pooled.

    (A) Bead-linked transposomes mediate the simultaneous fragmentation of gDNA and the addition of Illumina sequencing primers. (B) Reduced-cycle PCR amplification amplifies sequencing ready DNA fragments and adds indexes and adapters. (C) Sequencing-ready fragments are washed and pooled.

    How Illumina DNA Prep technology works

    Illumina DNA Prep chemistry uses an innovative bead-linked transposome complex to fragment genomic DNA and add adapter tag sequences in a single reaction step (tagmentation). After saturation with input DNA, the bead-linked transposome complex fragments a set number of DNA molecules. This bead-based saturation allows the flexibility to use a wide DNA input range and delivers consistent, uniform fragment size distribution that yields normalized libraries.

    Following tagmentation, a limited-cycle PCR step or a PCR-free ligation step adds Illumina DNA Prep–specific index adapter sequences to both ends of a DNA fragment, enabling dual-indexed sequencing of pooled libraries. A subsequent bead-based cleanup step then prepares libraries for use on any Illumina sequencing platform.

    Learn more about tagmentation

    Introducing Illumina DNA Prep with Enrichment

    This on-demand webinar introduces Illumina DNA Prep with Enrichment, a fast and flexible targeted sequencing solution for DNA in the Illumina library prep portfolio. You’ll learn how this solution delivers consistent insert sizes, uniform coverage, optimized performance, and highly reproducible data across all Illumina sequencing systems.

    View webinar

    Featured workflow: Whole-genome sequencing with Illumina DNA Prep

    Illumina DNA Prep is part of an integrated workflow for next-generation sequencing (NGS) that includes library preparation, high-quality sequencing, and simplified data analysis. Using on-bead tagmentation chemistry, Illumina DNA Prep saves time and reduces hands-on touchpoints to deliver one of the fastest library preparation solutions in the Illumina library prep portfolio. Library prep takes ~3.5 hours total, with 1–1.5 hours of hands-on time. 

    1
    Prep
    2
    Sequence
    3
    Analyze

    Illumina DNA Prep FAQ

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    Additional resources

    Whole-genome sequencing

    Sequence the entire genome, including coding and noncoding regions. Ideal for discovery applications.

    Target enrichment

    Select and sequence specific genes or genomic regions of interest using predesigned or custom designed panels for gene profiling studies.

    Amplicon sequencing

    Ultradeep sequencing of PCR amplicons with cost-effective analysis of up to hundreds of target genomic regions in one assay.

    Speak to a specialist

    Talk to an Illumina specialist to learn more about Illumina DNA Prep products.