Innovative library preparation
Save time and gain lab efficiencies with fast, flexible sequencing library preparation
Illumina DNA Prep products represent the latest revolution in Illumina library prep chemistry. Unique bead-linked transposome chemistry integrates DNA extraction, fragmentation, library preparation, and library normalization to deliver the fastest, most flexible whole-genome and targeted enrichment workflows in the Illumina library prep portfolio.
Each kit generates sequencing-ready libraries featuring consistent insert sizes, uniform coverage, and exceptional data quality, regardless of DNA input amount or type, for use in various applications.
(A) Bead-linked transposomes mediate the simultaneous fragmentation of gDNA and the addition of Illumina sequencing primers. (B) Reduced-cycle PCR amplification amplifies sequencing ready DNA fragments and adds indexes and adapters. (C) Sequencing-ready fragments are washed and pooled.
Illumina DNA Prep chemistry uses an innovative bead-linked transposome complex to fragment genomic DNA and add adapter tag sequences in a single reaction step (tagmentation). After saturation with input DNA, the bead-linked transposome complex fragments a set number of DNA molecules. This bead-based saturation allows the flexibility to use a wide DNA input range and delivers consistent, uniform fragment size distribution that yields normalized libraries.
Following tagmentation, a limited-cycle PCR step or a PCR-free ligation step adds Illumina DNA Prep–specific index adapter sequences to both ends of a DNA fragment, enabling dual-indexed sequencing of pooled libraries. A subsequent bead-based cleanup step then prepares libraries for use on any Illumina sequencing platform.
This on-demand webinar introduces Illumina DNA Prep with Enrichment, a fast and flexible targeted sequencing solution for DNA in the Illumina library prep portfolio. You’ll learn how this solution delivers consistent insert sizes, uniform coverage, optimized performance, and highly reproducible data across all Illumina sequencing systems.
Illumina DNA Prep is part of an integrated workflow for next-generation sequencing (NGS) that includes library preparation, high-quality sequencing, and simplified data analysis. Using on-bead tagmentation chemistry, Illumina DNA Prep saves time and reduces hands-on touchpoints to deliver one of the fastest library preparation solutions in the Illumina library prep portfolio. Library prep takes ~3.5 hours total, with 1–1.5 hours of hands-on time.
Illumina DNA Prep can be used in a wide range of applications, from sequencing large whole genomes to sequencing microbial species.
Illumina DNA Prep with Enrichment includes the same steps as Illumina DNA Prep but allows for targeted sequencing using custom, fixed, and exome panels in a final enrichment step.
Illumina DNA PCR-Free Prep removes the PCR step, eliminating PCR-induced bias. It is ideal for sensitive applications, such as human whole-genome sequencing.
Illumina DNA Prep includes the reagents needed for generating sequencing-ready libraries and magnetic sample purification beads for library cleanup steps. Index adapters must be purchased separately.
Illumina DNA Prep with Enrichment includes reagents needed for preparing and enriching sequencing-ready libraries. AMPure XP beads for library cleanup steps, index adapters, and your chosen enrichment oligo panel must be purchased separately.
Illumina DNA PCR-Free Prep includes the reagents needed for generating sequencing-ready libraries and magnetic sample purification beads for library cleanup steps. Sequencing primers, sequencing and indexing primers, and index adapters must be purchased separately.
Talk to an Illumina specialist to learn more about Illumina DNA Prep products.
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