AmpliSeq for Illumina Comprehensive Panel v3

Targeted DNA and RNA research panel investigating variants across 161 genes associated with a range of cancer types.Read More...
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Panel

AmpliSeq™ Comprehensive Panel v3 for Illumina®

20019109


Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103


Index Adapters

AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)

20019104

AmpliSeq™ CD Indexes Set A for Illumina®

20019105

AmpliSeq™ CD Indexes Set B for Illumina®

20019106

AmpliSeq™ CD Indexes Set C for Illumina®

20019107

AmpliSeq™ CD Indexes Set D for Illumina®

20019167

AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)

20031676

Ampliseq™ CD Indexes Large Volume for Illumina® (96 Indexes, 96 Samples)

20019108

配件产品

Ampliseq™ cDNA Synthesis for Illumina®

20022654

AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

AmpliSeq™ Library Equalizer for Illumina®

20019171

产品特色

The AmpliSeq for Illumina Comprehensive Panel v3 offers coverage of key cancer genes, including kinase domains and genes involved in DNA repair.

Relevant Gene Content
  • Target 161 unique cancer-associated genes
Fast, Streamlined Workflow
  • Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) input samples
Accurate Data
  • Detect somatic mutations down to 5% frequency using local or cloud-based analysis

The Comprehensive Panel v3 is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

The panel content spans hotspot regions, full-length genes*, copy number genes, and inter- and intragenic gene fusions. This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

*For this panel, Illumina defines full-length gene content as covering the majority of the gene’s coding regions, generally having > 90% in silico coverage and with most genes having > 99% coverage. Please refer to the provided product manifest file for further detail.

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
MiniSeq System 3 samples per run (high output; assumes minimum coverage of 500×) 2 × 150 bp
MiSeq System 3 samples per run with v3 reagents (assumes minimum coverage of 500×) 2 × 150 bp
NextSeq 550 System Samples per run: mid output: 16, high output: 48 (assumes minimum coverage of 500×) 2 × 150 bp

产品比较

AmpliSeq for Illumina Comprehensive Panel v3 TruSight Tumor 170 TruSight Tumor 15 AmpliSeq for Illumina Focus Panel AmpliSeq for Illumina Cancer Hotspot Panel v2
Assay Time 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Cancer Type Solid Tumor Solid Tumor Solid Tumor Solid Tumor Solid Tumor, 泛癌种
Content Specifications DNA and RNA targets for 161 oncogenes DNA and RNA targets for 52 oncogenes Hotspot regions of 50 genes with known associations to cancer
Description Somatic analysis research on hotspot and full-length targets of genes associated with solid tumor cancers. Somatic analysis research on 52 genes associated with solid tumor cancers. Somatic analysis research into hotspot regions of 50 cancer-related genes.
Hands-On Time < 1.5 hours < 1.5 hours < 1.5 hours
Input Quantity 1–100 ng (10 ng recommended per pool) 1–100 ng (10 ng recommended per pool) 1–100 ng (10 ng recommended per pool)
Method Amplicon Sequencing , 靶向DNA测序 , 靶向RNA测序 靶向DNA测序 , 靶向RNA测序 , 靶向富集 Amplicon Sequencing , 靶向DNA测序 Amplicon Sequencing , 靶向DNA测序 , 靶向RNA测序 Amplicon Sequencing , 靶向DNA测序
Multiplexing 96 dual index combinations 96 dual index combinations 96 dual index combinations
Nucleic Acid Type DNA, RNA DNA, RNA DNA DNA, RNA DNA
Specialized Sample Types FFPE Tissue FFPE Tissue, Low-Input Samples FFPE Tissue FFPE Tissue Blood, FFPE Tissue
Species Category 人类 人类 人类 人类 人类

Method-Specific Workflow Example

 

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