Instruments

MiniSeq Sequencing System specifications

Robust sequencing with a small footprint

Rapid turnaround, scalable throughput, and cost-effective benchtop sequencing with high quality scores

MiniSeq rendering

For every lab, everywhere

Access NGS with unrivaled simplicity and unthinkable speed

Meet the MiSeq i100 Series

Output per flow cell for various read lengthsa

MiniSeq Sequencing System

Read length MiniSeq System Mid Output Kit MiniSeq System Rapid Kit MiniSeq System High Output Kit
1 × 75 bp N/A N/A 1.65–1.875 Gb
2 × 75 bp N/A N/A 3.3–3.75 Gb
1 × 100 bp N/A 2 Gb N/A
2 × 150 bp 2.1–2.4 Gb N/A 6.6–7.5 Gb

a. Install specifications based on Illumina PhiX control library at supported cluster densities (129 –165 k/mm2 clusters passing filter). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter. All MiniSeq System kits are paired-end compatible.

Reads passing filter per flow cell

MiniSeq Sequencing System

Read length MiniSeq System Mid Output Kit MiniSeq System Rapid Kit MiniSeq System High Output Kit
Single-end reads 7–8M 20M 22–25M
Paired-end reads 14–16M N/A 44–50M

Quality scoresa

MiniSeq Sequencing System

Read length MiniSeq System Mid Output Kit MiniSeq System Rapid Kit MiniSeq System High Output Kit
1 × 75 bp N/A N/A > 85% bases higher than Q30 
2 × 75 bp N/A N/A > 85% bases higher than Q30 
1 × 100 bp N/A > 85% bases higher than Q30 N/A
2 × 150 bp > 80% bases higher than Q30 N/A > 80% bases higher than Q30

a. A quality score is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run.

Run timea

MiniSeq Sequencing System

Read length MiniSeq System Mid Output Kit MiniSeq System Rapid Kit MiniSeq System High Output Kit
1 × 75 bp N/A N/A 7 hr
2 × 75 bp N/A N/A 13 hr
1 × 100 bp N/A < 5 hr N/A
2 × 150 bp ~17 hr N/A ~24 hr

a. Includes cluster generation, sequencing, and base calling with quality scores on a MiniSeq System. Run times are based on Illumina PhiX control library at supported cluster densities. Performance may vary based on library type and quality, insert size, loading concentration, and other experimental factors.

Instrument specifications

MiniSeq system specifications

MiniSeq instrument specifications

  • Light emitting diode (LED)
    Wavelengths
    Green 510-525 nm
    Red 645-655 nm
  • Dimensions
    W x D x H
    45.6 cm × 48 cm × 51.8 cm
    (18.0 in × 18.9 in × 20.4 in)
  • Weight
    45 kg (99 lb)
  • Crated weight
    56.5 kg (125 lb)
View in-depth specs sheet (PDF)

Key technologies

Sequencing by synthesis chemistry

The MiniSeq System harnesses proven Illumina sequencing by synthesis (SBS) chemistry to deliver highly accurate data and robust performance for a broad range of applications. SBS chemistry uses reversible-terminator fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands, reading billions of sequences in parallel.

2-channel sequencing by synthesis

The MiniSeq System leverages 2-channel sequencing by synthesis (SBS) for faster data generation than the original Illumina 4-channel SBS technology, with the same high data accuracy.