This video shows how to use LNB1/2/3 plus additives (LNA1) to normalize the quantity of each library to ensure a uniform library representation in pooled libraries. Subscribe to the Illumina video channel http://www.youtube.com/subscription_center?add_user=IlluminaInc A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. View customer spotlight videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View Illumina webinars https://www.youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View Illumina support videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM LinkedIn: https://www.linkedin.com/company/illumina X: https://x.com/illumina Instagram: https://www.instagram.com/illuminainc/ Facebook: https://www.facebook.com/illuminainc TikTok: https://www.tiktok.com/@illuminainc #IlluminaLibraryPrep #BeadHandlingBestPractices #illumina 00:00 Introduction 00:08 Vortex library normalization beads (LNB) 01:32 Prepare LNA1 + LNB master mix 03:00 Add master mix and samples to MIDI, shake 03:10 Incubation steps 03:18 Remove supernatant from wells 03:51 Wash beads on magnetic stand with LNW1 05:50 Add elute solution to wells over pellet 06:40 Incubation steps 06:44 Transfer eluate to new PCR plate