This video shows how to use Sample Purification Beads (SPB) & Illumina Purification Beads (IPB) to purify cDNA or gDNA fragments and remove unwanted products and reaction components. Subscribe to the Illumina video channel youtube.com/subscription_center?add_user=IlluminaInc A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. View customer spotlight videos youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View Illumina webinars youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View Illumina support videos youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM LinkedIn: linkedin.com/company/illumina X: x.com/illumina Instagram: instagram.com/illuminainc Facebook: facebook.com/illuminainc TikTok: tiktok.com/@illuminainc #IlluminaLibraryPrep #BeadHandlingBestPractices #illumina 00:00 Introduction 00:08 Vortex SPB beads and add to a MIDI plate with samples 01:16 Incubation steps including magnetic plate 01:38 Tips for sample placement and pipetting 02:00 Remove supernatant from wells 02:52 Wash beads on magnetic stand with 80% ethanol 04:04 Tips for best magnetic stand bead visibility 04:37 Add Resuspension Buffer (RSB) to wells over bead pellet – Method 1 – single channel pipette 05:21 Add Resuspension Buffer (RSB) to wells over bead pellet – Method 2 – multichannel with strip tube 06:24 Incubation steps 06:34 Transfer eluate to new PCR plate