Assaying genome-wide chromatin accessibility with ATAC-seq

Understanding the epigenetic regulation of gene expression in different cells and tissues is a key question in systems biology. Since it was first reported in 2013, Assay for Transposase Accessible Chromatin using sequencing (ATAC-seq) has been widely adopted as a sensitive, robust tool for genome-wide chromatin accessibility profiling. ATAC-seq has since been adapted for single-cell analysis to study cell type-specific chromatin accessibility in different tissues with heterogeneous cell populations.

This webcast will show participants how to use ATAC-seq and how it fits in with other chromatin accessibility profiling methods. First, William Greenleaf will discuss the development of ATAC-seq, its use, and adaptation to single cells. Bing Ren and Sebastian Preissl will then discuss single-cell ATAC-seq using combinatorial indexing, and application to brain, hearts and other tissues. These presentations will be followed by a live discussion with the speakers.

William Greenleaf
Associate Professor
Stanford University

Bing Ren
Professor of Cellular and Molecular Medicine
University of California, San Diego

Sebastian Preissl
Associate Director, single-cell genomics
Center for Epigenomics, UCSD