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Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.
A flexible and streamlined NGS library prep kit that enables various public health surveillance and microbial research applications.
Assay time
Hands-on time
Input quantity
Illumina Microbial Amplicon Prep (iMAP) is an amplicon-based library preparation kit built on the same chemistry as COVIDSeq. Library preparation is fast and simple, with libraries compatible with nearly all Illumina sequencing systems. It can be used for various infectious disease, public health surveillance, and microbial research applications, including viral whole-genome sequencing, antimicrobial resistance marker analysis, bacterial and fungal identification, and more.
Get sequencing results with exceptional data quality and accuracy at any scale with low hands-on time.
The kit can be used with custom, published, or commercially available primer sets (primer oligos are not included) and is compatible with RNA or DNA extracted from sources such as cultures, swabs (nasopharyngeal, skin, and nasal), wastewater, and more. Analysis can be performed using the DRAGEN Targeted Microbial App on Basespace Sequence Hub with pre-loaded targets. Files can also be easily uploaded for custom analyses.
*Protocols provided for informational purposes only. This does not imply the protocols are validated or supported by Illumina.
Assay time | < 9 hr |
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Automation capability | Liquid handling robots |
Description | Illumina Microbial Amplicon Prep (IMAP) is an amplicon-based library preparation solution that includes RT-PCR, library prep, and indexes for 48 samples. It is built on the same chemistry as COVIDSeq and can be used with DNA and RNA samples. Oligo primers are not provided in the kit and are sourced separately. |
Hands-on time | ~3 hr for 48 samples |
Input quantity | Varies depending on sample source |
Instruments | MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System |
Mechanism of action | Multiplex PCR |
Method | Amplicon sequencing |
Nucleic acid type | DNA, RNA |
Sample type details | Works with a wide variety of sample types from nasal swabs to wastewater |
Species category | Other, Yeast, Virus, Bacteria |
Technology | Sequencing |
Variant class | Single nucleotide polymorphisms (SNPs), Single nucleotide variants (SNVs) |
Illumina Microbial Amplicon Prep
Learn about wastewater surveillance with NGS for community-level monitoring of infectious diseases, mutation tracking, and variant trend tracking.
Genomic surveillance of infectious diseases
NGS supports effective genomic surveillance strategies through identification of novel strains of coronavirus and other emerging infectious disease agents.
Ultra-deep sequencing of PCR amplicons enables analysis of specific genomic regions of interest. Learn more about amplicon sequencing and find comprehensive solutions.
In this video, Illumina Scientist Jeff Koble provides an overview of the Illumina Microbial Amplicon Prep assay, detailing tips and tricks to help ensure successful preparation of libraries.
Illumina Bioinformatics Scientist Soo Bin Kwon demonstrates how DRAGEN Target Microbial enables comprehensive analysis of microbial targets sequenced with IMAP.
Illumina Microbial Amplicon Prep
20097857
48-sample amplicon kit containing cDNA conversion, library prep, and indexes. Primers are not included.
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