NextSeq 550Dx Instrument specifications

The reliability and consistency of a clinical instrument

Delivering high-quality, reliable data for diagnostic testing using proven SBS chemistry

NextSeq 550Dx instrument render

Output per flow cell

NextSeq 550Dx Instrument

Read length NextSeq 550Dx High-Output Reagent Kit v2.5 (300 cycles)
2 × 150 bp 120 Gb

Reads passing filter per flow cell

NextSeq 550Dx Instrument

  NextSeq 550Dx High-Output Reagent Kit v2.5 (300 cycles)
Paired-end reads ≥ 600M

Quality scoresa

NextSeq 550Dx Instrument

Read length NextSeq 550Dx High-Output Reagent Kit v2.5 (300 cycles)
2 × 150 bp > 75% bases higher than Q30

a. A quality score is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run.

Run timea

NextSeq 550Dx Instrument

Read length NextSeq 550Dx High-Output Reagent Kit v2.5 (300 cycles)
2 × 150 bp ≤ 35 hr

Total overall accuracya

NextSeq 550Dx Instrument

Somatic variant caller ≥ 99.98%
Germline variant caller ≥ 99.99%

a. Using a representative assay designed to query a variety of genes covering approximately 13K bases across 23 different chromosomes and containing wide GC content (18%–87%), indels (up to 25 base pairs), and homopolymers (7–13 nucleotides).

Instrument specifications

NextSeq 550Dx instrument specifications
  • Output range
    16.25 - 120 Gb
  • Reads per run
    130 - 400 million
  • Max read length
    2 × 150 bp
  • Run time
    12 - 30 hr
  • Light emitting diode (LED)
    Wavelengths

    (laser diode 780 nm, Class IIIb)
    Green 510–525 nm, red 645–655 nm
  • Dimensions
    W × D × H
    54 cm × 69 cm × 58 cm
    (21.3 in × 27.2 in × 22.8 in)
  • Weight
    84.4 kg (186 lb)
  • Crated weight
    163 kg (360 lb)

Specifications shown here are for IVD use. View research only specifications, applicable when running the NextSeq 550Dx Instrument in Research Mode.
 

Key technologies

Intended Use for the NextSeq 550Dx Instrument

United States:

The NextSeq 550Dx Instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue when used with in vitro (IVD) diagnostic assays. The NextSeq 550Dx Instrument is not intended for whole genome or de novo sequencing. The NextSeq 550Dx Instrument is to be used with specific registered, certified, or approved in vitro diagnostic reagents and analytical software.

European Union:

The NextSeq 550Dx instrument is intended for sequencing of DNA libraries when used with in vitro diagnostic assays. The NextSeq 550Dx instrument is to be used with specific registered, certified, or approved in vitro diagnostic reagents and analytical software.