Illumina Microbial Amplicon Prep—Influenza A/B

A streamlined whole-genome NGS library prep kit for high-impact research and public health surveillance of influenza A and B viruses.

Overview

Illumina Microbial Amplicon Prep—Influenza A/B is an amplicon-based library preparation kit built on the same chemistry as the COVIDSeq assay. Library preparation is fast and easy, generating libraries compatible with nearly all Illumina sequencing systems. This kit enables whole-genome sequencing of influenza A/B viruses for public health surveillance and influenza virus research.

Expand your public health surveillance and influenza virus research capabilities

Get sequencing results with exceptional data quality and accuracy at any scale with fast turnaround times.

Key features

  • Multiplexed, automation-friendly RT-PCR and tagmentation-based workflow

  • Simplified data analysis and reporting with the DRAGEN Targeted Microbial App on BaseSpace Sequence Hub 

  • Excellent performance with RNA from a range of sample types, including cultures and nasal swabs

This kit provides exceptional coverage across influenza A/B virus genomes, addressing sequencing challenges related to high genomic variability. In addition, achieve rapid, unbiased, and accurate influenza virus sequencing and characterization to support high-impact genomic surveillance and research efforts.

Specifications

Applications

Example workflow

1
Prep

Illumina Microbial Amplicon Prep—Influenza A/B

Related applications and methods

Compare

Illumina Microbial Amplicon Prep—Influenza A/B Illumina Microbial Amplicon Prep Respiratory Pathogen ID/AMR Enrichment Panel Kit Illumina Respiratory Virus Enrichment Kit
Automation capability Liquid handling robots Liquid handling robots Liquid handling robots Liquid handling robots
Instruments MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System MiSeq System, NextSeq 550 System, MiniSeq System MiSeq System, NextSeq 550 System, NextSeq 2000 System, MiniSeq System
Method Amplicon sequencing, Targeted RNA sequencing Amplicon sequencing Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment
Nucleic acid type RNA DNA, RNA DNA, RNA DNA, RNA
Species category Virus Other, Yeast, Virus, Bacteria Fungal, Virus, Bacteria Human, Virus
Strand specificity Non-stranded
Technology Sequencing Sequencing Sequencing Sequencing
Variant class Single nucleotide polymorphisms (SNPs), Single nucleotide variants (SNVs), Insertions-deletions (indels) Single nucleotide polymorphisms (SNPs), Single nucleotide variants (SNVs) Single nucleotide polymorphisms (SNPs), Single nucleotide variants (SNVs), Insertions-deletions (indels)

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Resources

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Fast, flexible library prep with IMAP

In this video, Illumina Scientist Jeff Koble provides an overview of the Illumina Microbial Amplicon Prep assay, detailing tips and tricks to help ensure successful preparation of libraries.

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Simple and efficient IMAP analysis with DRAGEN Targeted Microbial App

Illumina Bioinformatics Scientist Soo Bin Kwon demonstrates how DRAGEN Target Microbial enables comprehensive analysis of microbial targets sequenced with IMAP.

Illumina® Microbial Amplicon Prep—Influenza A/B (48 samples)

20106305

Complete amplicon-prep based library prep kit for whole genome sequencing of Influenza A and B virus strains. Amplicon sequencing technology is based on well established COVIDSeq but optimized for Influenza A/B viral sequencing. Each kit provides enough reagents for 48 samples and includes library prep reagents, an influenza A/B targeting primer pool, and an index plate with 48 unique dual indexes.

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