Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI) from FFPE tissue, with added scalability.
Assay time
Hands-on time
Input quantity
Now supported on the NovaSeq X Series, NextSeq 1000 and NextSeq 2000
TruSight Oncology 500 High-Throughput (HT) is a highly scalable pan-cancer, next-generation sequencing (NGS) assay that enables in-house comprehensive genomic profiling (CGP) from FFPE tissue.
Evaluate > 500 pan-cancer biomarkers aligned with guidelines and clinical trials to increase chances of finding a positive biomarker with a single assay.
Get results in 4–5 days using manual or automated workflows integrated with ready-to-use DRAGEN secondary analysis pipelines via on-premises server or in the cloud with Illumina Connected Analytics. Access an integrated interpretation report through Illumina Connected Insights† or Velsera Clinical Genomics Workspace (CGW).
* Automation available on the Beckman Coulter iSeries or the Hamilton Microlab STAR. Contact Illumina Sales for more information.
†Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.
Identify relevant biomarkers for clinical research with the TruSight Oncology 500 product line.
TruSight Oncology 500 High-Throughput
Batch up to 960 samples at a time while using the same panel content and tissue input type as TruSight Oncology 500.
Assess relevant DNA and RNA cancer biomarkers from FFPE tumor tissue, now including HRD through an accessory kit to evaluate the Genomic Instability Score (GIS).‡
Analyze circulating tumor DNA (ctDNA) in blood plasma via liquid biopsy with similar DNA panel content as TruSight Oncology 500.
‡ HRD is only available with the addition of the TruSight Oncology 500 HRD kit to TruSight Oncology 500. Not available in Japan.
§ NextSeq 1000, NextSeq 2000, NovaSeq 6000Dx System (in research mode) and NovaSeq X Series require a separate, stand-alone DRAGEN server for secondary analysis.
| Assay time | 4–5 days from sample to results |
|---|---|
| Automation capability | Liquid handling robot(s) |
| Automation details | Explore available automation methods |
| Cancer type | Pan-cancer, Solid tumor |
| Content specifications | Targeted sequencing of DNA from 523 genes of interest and RNA from 55 genes, for a total of 1.94Mb panel size. MSI and TMB measurement included. |
| Description | A high-throughput comprehensive NGS assay to identify key biomarkers in guidelines and >1K clinical trials from a streamlined workflow using the NextSeq 1000, NextSeq 2000, NovaSeq 6000, NovaSeq 6000Dx (in research mode) or the NovaSeq X Series. Includes coverage of immuno-oncology biomarker TMB and MSI. |
| Hands-on time |
~2.5 hr for automated workflow ~10.5 hr for manual workflow |
| Input quantity | 40 ng DNA and/or 40–80 ng RNA |
| Instruments | NextSeq 2000 System, NextSeq 1000 System, NovaSeq X System, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System |
| Method | Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment |
| Multiplexing |
NextSeq 1000 and 2000: P2 flow cell 8 samples, P3 flow cell 24 samples, P4 flow cell 36 samples NovaSeq 6000/Dx: SP flow cell 16 samples, S1 flow cell 32 samples, S2 flow cell 72 samples, S4 flow cell 192 samples NovaSeq X Series*: 1.5B cartridge 32 samples, 10B cartridge 192 samples, 25B 480 samples |
| Nucleic acid type | DNA, RNA |
| Sample throughput | NextSeq 1000 and 2000: 8-36 samples/run, NovaSeq 6000/Dx: 16-192 samples/run, NovaSeq X: 32-480 samples/run, NovaSeq X Plus: 32-960 samples/run |
| Specialized sample types | FFPE tissue |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Gene fusions, Somatic variants, Novel transcripts, Structural variants, Transcript variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) |
*Throughput shown is for the single flow cell NovaSeq X Instrument. NovaSeq X Plus offers a dual flow cell system with twice the capacity listed.
To run TruSight Oncology 500 High-Throughput (TSO 500 HT) you need:
To analyze with the DRAGEN variant calling pipeline you need:
To generate a final report, you need:
§NextSeq 1000, NextSeq 2000, NovaSeq 6000Dx (in research mode) and NovaSeq X Series require a separate, stand-alone DRAGEN server for secondary analysis.
†Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.
TruSight Oncology 500 High-Throughput enables comprehensive genomic profiling of FFPE tumor samples to assess a wide range of biomarkers at increased scalability, from 8–480 samples per flow cell.
TruSight Oncology 500 High-Throughput assay
** Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| NextSeq 1000 System, NextSeq 2000 System | 8 samples per run (P2 flow cell), 800M paired-end reads, 3,500× coverage 24 samples per run (P3 flow cell), 2.4B paired-end reads, 3,500× coverage 36 samples per run (P4 flow cell), 3.6B paired-end reads, 3,500× coverage |
2 x 101 bp |
| NovaSeq 6000 System | 16 samples per run (SP flow cell), 100M paired-end reads, 3,500× coverage 32 samples per run (S1 flow cell), 100M paired-end reads, 3,500× coverage 72 samples per run (S2 flow cell), 100M paired-end reads, 3,500× coverage 192 samples per run (S4 flow cell), 100M paired-end reads, 3,500× coverage |
2 × 101 bp |
| NovaSeq 6000Dx in Research Mode | 16 samples per run (SP flow cell), 100M paired-end reads, 3,500× coverage 32 samples per run (S1 flow cell), 100M paired-end reads, 3,500× coverage 72 samples per run (S2 flow cell), 100M paired-end reads, 3,500× coverage 192 samples per run (S4 flow cell), 100M paired-end reads, 3,500× coverage |
2 × 101 bp |
| NovaSeq X System | 32 samples per run (1.5B flow cell), 3.2B paired-end reads, 3,500× coverage 192 samples per run (10B flow cell), 20B paired-end reads, 3,500× coverage 480 samples per run (25B flow cell), 52B paired-end reads, 3,500× coverage *Throughput shown is for the single flow cell NovaSeq X Instrument. |
2 × 101 bp |
Reduce hands-on time and minimize errors with high- and low-throughput walk-away automation protocols available through Illumina automation partners.
Next-generation sequencing enables immuno-oncology researchers to gain insights into immunotherapy response factors and tumor immune evasion mechanisms.
Pathology and clinical cancer research
Our clinical cancer research solutions deliver accurate genomic information, and enable labs to analyze multiple genes in a single test.
| TruSight Oncology 500 High-Throughput | TruSight Oncology 500 | TruSight Oncology 500 ctDNA v2 | |||
|---|---|---|---|---|---|
| Assay time | 4–5 days from sample to results | 4–5 days from sample to results | 3-4 days from purified nucleic acid to variant report | ||
| Automation capability | Liquid handling robot(s) | Liquid handling robot(s) | Liquid handling robot(s) | ||
| Automation details | Explore available automation methods | Explore available automation methods | Explore available automation methods | ||
| Cancer type | Pan-cancer, Solid tumor | Pan-cancer, Solid tumor | Pan-cancer, Solid tumor | ||
| Content specifications | Targeted sequencing of DNA from 523 genes of interest and RNA from 55 genes, for a total of 1.94Mb panel size. MSI and TMB measurement included. | Targeted sequencing of DNA from 523 genes and RNA from 55 genes for a total of 1.94 Mb panel size. MSI and TMB measurement included. The optional TruSight Oncology 500 HRD kit (not available in Japan) content includes coverage of ~25K SNPs to assess homologous recombination deficiency through a comprehensive genomic instability score (LOH+TAI+LST) powered by Myriad Genetics. |
Targeted selection of 523 genes (full coding sequence) for a total of 1.94Mb panel size. • Immuno-oncology biomarker coverage: TMB and MSI • Guideline coverage: Broad coverage of key guidelines for multiple solid tumor types • Clinical trial coverage: Over 600 clinical trials (based on Velsera clinical knowledgebase, as of February 2023) |
||
| Description | A high-throughput comprehensive NGS assay to identify key biomarkers in guidelines and >1K clinical trials from a streamlined workflow using the NextSeq 1000, NextSeq 2000, NovaSeq 6000, NovaSeq 6000Dx (in research mode) or the NovaSeq X Series. Includes coverage of immuno-oncology biomarker TMB and MSI. | Assay that enables comprehensive genomic profiling from FFPE tissue and runs on the NextSeq 550 System or NextSeq 550Dx Instrument (in Research Mode) and can batch up to eight samples at a time. | Provides a noninvasive research method for comprehensive genomic profiling of liquid biopsy samples (ctDNA from blood plasma). This liquid biopsy approach provides insights about intra- and inter-tumor heterogeneity using a minimally invasive sample collection approach to complement tissue-based CGP. | ||
| Hands-on time |
~2.5 hr for automated workflow ~10.5 hr for manual workflow |
~2.5 hr for automated workflow ~10.5 hr for manual workflow |
~1.5 hr for automated workflow ~2.5 hr for manual workflow |
||
| Input quantity | 40 ng DNA and/or 40–80 ng RNA | 40 ng DNA and/or 40 ng RNA | 20 ng cfDNA (4 ml of plasma) | ||
| Instruments | NextSeq 2000 System, NextSeq 1000 System, NovaSeq X System, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System | NextSeq 550 System, NextSeq 550Dx in Research Mode, NextSeq 500 System | NovaSeq X System, NovaSeq 6000 System | ||
| Method | Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment | Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment | Targeted DNA sequencing, Target enrichment | ||
| Multiplexing |
NextSeq 1000 and 2000: P2 flow cell 8 samples, P3 flow cell 24 samples, P4 flow cell 36 samples NovaSeq 6000/Dx: SP flow cell 16 samples, S1 flow cell 32 samples, S2 flow cell 72 samples, S4 flow cell 192 samples NovaSeq X Series*: 1.5B cartridge 32 samples, 10B cartridge 192 samples, 25B 480 samples |
Up to 8-plex |
NovaSeq X Series: 4 samples on 1.5B flow cell, 24 samples on 10B flow cell NovaSeq 6000 System: 8 samples on S2 flow cell, 24 samples on S4 flow cell, 192 indexes maximum |
||
| Nucleic acid type | DNA, RNA | DNA, RNA | DNA | ||
| Sample throughput | NextSeq 1000 and 2000: 8-36 samples/run, NovaSeq 6000/Dx: 16-192 samples/run, NovaSeq X: 32-480 samples/run, NovaSeq X Plus: 32-960 samples/run | 8 samples/run | 4-48 samples/run | ||
| Specialized sample types | FFPE tissue | FFPE tissue | Circulating tumor DNA, Blood | ||
| Species category | Human | Human | Human | ||
| Technology | Sequencing | Sequencing | Sequencing | ||
| Variant class | Gene fusions, Somatic variants, Novel transcripts, Structural variants, Transcript variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) | Gene fusions, Transcript variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs), Loss of heterozygosity (LOH), Somatic variants, Novel transcripts, Single nucleotide polymorphisms (SNPs), Structural variants | Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) |
The information in this table shows examples of concordance between TruSight Oncology 500 and TruSight Oncology 500 High-Throughput and is not a comprehensive list of the fusions and splice variants detected. These data were generated with a Local App pipeline (not DRAGEN software).
High concordance between NovaSeq X Series and NovaSeq 6000 for TSO 500 HT
Hear how customers at Intermountain Precision Genomics enabled CGP and scaled up their workflow with automation.
TruSight Oncology 500 DNA High-Throughput (48 Samples)
20040765
Includes reagents for preparing and enriching up to 48 DNA samples. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.
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TruSight Oncology 500 DNA High-Throughput (144 Samples)
20040767
Includes reagents for preparing and enriching up to 144 DNA samples. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.
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TruSight Oncology 500 DNA/RNA High-Throughput (24 Samples)
20040764
Includes reagents for preparing and enriching up to 24 DNA and RNA samples. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.
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TSO 500 DNA/RNA HT (24 Spl) plus Illumina Connected Insights Software
20119460
Includes library prep and enrichment reagents plus data interpretation reports (through ICI).
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TruSight Oncology 500 DNA/RNA High-Throughput (72 Samples)
20040766
Includes reagents for preparing and enriching up to 72 DNA and RNA samples. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.
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TruSight Oncology 500 DNA/RNA High-Throughput Kit (24 Samples), plus Velsera
20040768
Includes reagents for preparing and enriching up to 24 DNA and RNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.
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TruSight Oncology 500 DNA High-Throughput Kit (48 Samples), plus Velsera
20040769
Includes reagents for preparing and enriching up to 48 DNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.
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TruSight Oncology 500 DNA/RNA High-Throughput Kit (72 Samples), plus Velsera
20040770
Includes reagents for preparing and enriching up to 72 DNA and RNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.
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TruSight Oncology 500 DNA High-Throughput Kit (144 Samples), plus Velsera
20040771
Includes reagents for preparing and enriching up to 144 DNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase index anchors and NovaSeq 6000 sequencing reagents separately.
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TruSight Oncology 500 High-Throughput DNA for Automation (64 Samples)
20049283
Includes reagents for preparing and enriching up to 64 DNA samples. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation.
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TruSight Oncology 500 High-Throughput DNA/RNA for Automation (32 Samples)
20049282
Includes reagents for preparing and enriching up to 32 DNA and RNA samples. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation.
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TSO500 DNA/RNA HT Auto (32 spls)+ plus Illumina Connected Insights Software
20119461
Includes library prep and enrichment reagents plus data interpretation reports (through ICI).
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TruSight Oncology 500 High-Throughput DNA for Automation (64 Samples), Plus Velsera
20049277
Includes reagents for preparing and enriching up to 64 DNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation.
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Discounts:
TruSight Oncology 500 High-Throughput DNA/RNA for Automation (32 Samples), Plus Velsera
20049276
Includes reagents for preparing and enriching up to 32 DNA and RNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation.
List Price:
Discounts:
TruSight Oncology 500 High-Throughput DNA for Automation (144 Samples)
20049285
Includes reagents for preparing and enriching up to 144 DNA samples. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation.
List Price:
Discounts:
TruSight Oncology 500 High-Throughput DNA for Automation (144 Samples), Plus Velsera
20049279
Includes reagents for preparing and enriching up to 144 DNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation.
List Price:
Discounts:
TruSight Oncology 500 High-Throughput DNA/RNA for Automation (72 Samples)
20049284
Includes reagents for preparing and enriching up to 72 DNA and RNA samples. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation.
List Price:
Discounts:
TruSight Oncology 500 High-Throughput DNA/RNA for Automation (72 samples) + Velsera
20049278
Includes reagents for preparing and enriching up to 72 DNA and RNA samples and data interpretation reports using Pierian Clinical Genomics Workspace. Purchase NovaSeq 6000 sequencing reagents separately. Intended for use with automation.
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IDT® for Illumina® UMI DNA/RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples)
20034701
UMI DNA Index Anchors (Plate = 20027219, Box = 20032119) + Nextera Compatible Unique Dual Index A (Sales Kit = 20027213, Plate = 20025019, Box = 20026121)
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IDT® for Illumina® UMI DNA/RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples)
20034702
UMI DNA Index Anchors (Plate = 20027219, Box = 20032119) + Nextera Compatible Unique Dual Index B (Sales Kit = 20027214, Plate = 20025080, Box = 20026930)
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IDT for Illumina UMI DNA/DNA Index Anchors Set A for Automation
20066404
Includes one box of 96 IDT for Illumina - UMI DNA/RNA UD Indexes Set A for Automation sufficient for labeling 96 samples and one box of 96 IDT for Illumina - UMI DNA Index Anchors for Automation.
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IDT for Illumina UMI DNA/DNA Index Anchors Set B for Automation
20063213
Includes one box of 96 IDT for Illumina - UMI DNA/RNA UD Indexes Set B for Automation sufficient for labeling 96 samples and one box of 96 IDT for Illumina - UMI DNA Index Anchors for Automation.
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NovaSeq™ X Series 1.5B Reagent Kit (200 Cyc)
20104704
This reagent kit provides one NovaSeq X Series 1.5B flow cell (with 2- lanes) and reagent consumables to support a single flow cell 200 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq X Series 10B Reagent Kit (200 Cycle)
20085595
This reagent kit provides one NovaSeq X Series 10B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 200 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq™ X Series 25B Reagent Kit (300 Cyc)
20104706
This reagent kit provides one NovaSeq X Series 25B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 300 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq 6000 S4 Reagent Kit v1.5 (200 cycles)
20028313
Includes one S4 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 200-cycle run on the NovaSeq 6000 System.
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NovaSeq 6000 S2 Reagent Kit v1.5 (200 cycles)
20028315
Includes one S2 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 200-cycle run on the NovaSeq 6000 System.
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NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycles)
20028318
Includes one S1 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 200-cycle run on the NovaSeq 6000 System.
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NovaSeq 6000 SP Reagent Kit v1.5 (200 cycles)
20040719
Includes one SP flow cell on,e buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 200-cycle run on the NovaSeq 6000 System.
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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (200 Cycles)
20100986
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 400M single reads).
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NextSeq™ 2000 P3 XLEAP-SBS™ Reagent Kit (200 Cycles)
20100989
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 1.2B single reads).
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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (200 Cycles)
20100993
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).
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Trusight Oncology 500 HT Training (DNA) - Customer Site
20042220
Three-day, hands-on instruction at customer site to familiarize users with TruSight Oncology 500 HT (DNA only). Course provides hands-on training in library preparation, enrichment, sequencing, and data analysis for a maximum of four trainees. NOTE: The short description should be updated as well. We no longer use "TST". It should be consistent with others and say "TSO 500."
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Trusight Oncology 500 HT Training (DNA and RNA) - Customer Site
20042219
3.5-day, hands-on instruction at customer site to familiarize users with TruSight Oncology 500 HT (DNA and RNA). Course provides hands-on training in library preparation, enrichment, sequencing, and data analysis for a maximum of four trainees. NOTE: The short description should be updated as well. We no longer use "TST". It should be consistent with others and say "TSO 500."
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Connected Insights Training - Remote
20092376
Illumina Connected Insights Training - Remote includes five (5) hours of product training delivered virtually.
Informatics Professional Services
20071787
Professional Services rendered for Illumina informatics products and solutions, which may be defined by a pre-determined scope or statement of work.
Illumina DRAGEN Server v3
20040619
Includes Avance Exchange support for the first year. Requires purchase of annual DRAGEN license.
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Illumina DRAGEN Server v4
20051343
Includes Advance Exchange support for the first year. Requires purchase of annual DRAGEN license.
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ICA Basic Annual Subscription
20044874
Illumina Connected Analytics (ICA) Basic Annual Subscription. This product includes 1 year of access to ICA Basic, including sequencing instrument connectivity, data management capabilities, and access to pre-packaged analysis tools.
ICA Professional Annual Subscription
20044876
Illumina Connected Analytics (ICA) Professional Annual Subscription. This product includes 1 year of access to ICA, including sequencing instrument connectivity, data management capabilities, access to pre- packaged tools, and the ability to create customized workflows composed of tools, pipelines, data warehouses, and notebooks.
ICA Enterprise Annual Subscription
20038994
Illumina Connected Analytics (ICA) Enterprise Annual Subscription. This product includes 1 year of access to ICA Enterprise, including sequencing instrument connectivity, data management capabilities, custom and pre-packaged analysis tools, and the Base module for data warehousing and mining. ICA Enterprise also includes optional HIPAA BAA (US-only), single sign-on (SSO), and a service level agreement (SLA).
ICA Enterprise Srvc & Compliance Add-on
20066830
Illumina Connected Analytics (ICA) Compliance enables single sign-on (SSO), multi-factor authentication (MFA), HIPAA BAA (US-only), and a Service Level Agreement (SLA) for an ICA Basic Annual Subscription.
Illumina Analytics - 1 iCredit
20042038
iCredits are used for data storage and analysis on either BaseSpace Sequence Hub or Illumina Connected Analytics.
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Illumina Analytics Starter Pack - 1,000 iCredits
20042039
iCredits are used for data storage and analysis on either BaseSpace Sequence Hub or Illumina Connected Analytics.
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Illumina Analytics - 5,000 iCredits
20042040
iCredits are used for data storage and analysis on either BaseSpace Sequence Hub or Illumina Connected Analytics.
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Illumina Analytics - 50,000 iCredits
20042041
iCredits are used for data storage and analysis on either BaseSpace Sequence Hub or Illumina Connected Analytics.
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Illumina Analytics - 100,000 iCredits
20042042
iCredits are used for data storage and analysis on either BaseSpace Sequence Hub or Illumina Connected Analytics.
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Insights - Annual Subscription
20090137
Annual subscription to Illumina Connected Insights platform.
Illumina Connected Insights – Oncology Genome Equivalent Sample - VCF
20090138
Illumina Connected Insights pre-paid oncology samples on per genome equivalent basis starting from VCF: one genome is equivalent to 2 exomes, 3 large and 6 small panel samples. Any unused samples will automatically roll over provided that the annual subscription to Illumina Connected Insights (20090137) is renewed on an annual basis. Access to a set of oncology knowledge bases is included.
Insights - Annual Subscription
20090137
Annual subscription to Illumina Connected Insights platform.
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The primary difference between the assays is scale. TruSight Oncology 500 HT runs on a variety of sequencers: NextSeq 1000 and 2000, NovaSeq 6000, NovaSeq 6000Dx (in Research Mode), and the NovaSeq X Series. Sample batching can range from 8-960 samples at a time, providing scalability to higher sample throughput. TruSight Oncology 500 runs on the NextSeq 550 System or NextSeq 550Dx Instrument (in Research Mode) and can batch up to 8 samples at a time. See comparison table on this page for other differences.
Yes, you can automate the TSO 500 HT workflow with Illumina Qualified automation methods. Illumina Qualified methods are available on the Beckman Coulter Life Sciences i7 and the Hamilton STAR (MOA configuration) liquid handling robots. Illumina Qualified methods are co-developed by Illumina and the automation partner. Automation specific library prep kits are offered by Illumina to accommodate the reagent volume required by automation platforms.
TruSight Oncology 500 HRD is not currently validated for use with TruSight Oncology 500 High-Throughput. Please reach out to your local Illumina sales or support representatives for information on alternative solutions. TruSight Oncology 500 HRD is not available in Japan.
The Illumina DRAGEN secondary analysis platform delivers greater variant calling accuracy at twice the speed of non-DRAGEN options.1 Increased accuracy provides better performance for sample QC, complex variants, small variants, fusions, and splice variants, as well as exon-level CNV calling for BRCA1/BRCA2. Illumina will continue to bring innovative features to DRAGEN TruSight Oncology 500 pipelines.
Yes, you can perform analysis with your own software. However, Illumina will not be able to directly provide technical support in this case.
Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
A high-performance, fast, and reliable human whole-exome sequencing solution that includes a comprehensive, up-to-date exome enrichment panel.
A fast, flexible, and scalable cfDNA library preparation kit for highly sensitive mutation detection
Reagent kits for the NovaSeq X Series include a reagent cartridge, buffer cartridge, flow cell, lyo insert, pre-load buffer, and library tube strip.
Reagent kits for the NovaSeq 6000 System consisting of ready-to-use pre-filled reagent cartridges and flow cells.
Enable CGP with a pan-cancer panel capable of detecting key IO gene signatures (TMB, MSI) plus all main variant classes from ctDNA in blood plasma.
Target 1385 cancer genes for gene expression, variant, and fusion detection studies in many RNA sample types, including blood, bone marrow, and FFPE tissue.
References
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