Illumina 5-base solution
One assay.
Dual insights.
Detect five DNA bases in a single assay while delivering concurrent genetic and epigenetic insights
The new standard in methylation sequencing
The Illumina 5-base solution enables direct detection of five DNA bases—adenine (A), thymine (T), cytosine (C), guanine (G), and 5-methylcytosine (5mC) at cytosine-guanine dinucleotide (CpG) sites—in a single sequencing workflow. By preserving nucleotide diversity and maintaining library complexity, the Illumina 5-base solution supports high sequence alignment rates and information-rich data. The optimized workflow integrates one library prep, one sequencing run, and one analysis pipeline to deliver dual omic insights without added assay complexity.
Key features and benefits
Product data highlights
Testimonial
DNA and methylation: Dual insights, single assay
Bekim Sadikovic, PhD, Chief Scientific Officer at EpiSign Inc and Helge Lubenow, PhD, Chief Executive Officer at Heidelburg Epignostix discuss how the Illumina 5-base solution reveals hidden mechanisms of disease in the genome and epigenome in a single, streamlined, multiomic assay.
DNA and methylation: Dual insights, single assay
Applications
The Illumina 5-base solution is well-suited for applications that benefit from concurrent methylation and genetic variant information, particularly where DNA quality, complexity, and interpretability matter. Applications include:
* cfDNA, cell-free DNA; ctDNA, circulating tumor DNA; EWAS, epigenome-wide association studies; FFPE, formalin-fixed paraffin-embedded; NGS, next-generation sequencing.
Workflow highlights
Innovative chemistry preserves DNA integrity
≥ 6 hours library preparation time
~1 hour dual data analysis for a 30× genome
5-base solution workflow
The streamlined 5-base workflow includes optimized whole-genome library prep that takes ~6 hours from DNA to sequencing system and offers easy-to-use analysis for dual DNA and methylation annotations and visualizations.
Library prep
A single assay for comprehensive discovery of the 5-base genome (A, T, G, C, and 5mC), providing dual insights into the whole genome and methylome.
Sequencing
Perform powerful high-throughput sequencing studies. Match data output and time to results to project needs.
Advanced chemistry, optics, and informatics combine to deliver exceptional sequencing speed and data quality, outstanding throughput, and scalability.
Data analysis
Accurate, comprehensive, and efficient secondary analysis of next-generation sequencing (NGS) data.
A cloud-based multiomic analysis and interpretation software, enabling sample to insight workflow with visualizations, scalability, and secure data management.
The streamlined 5-base workflow includes optimized target enrichment library prep and easy-to-use analysis for dual DNA and methylation annotations and visualizations of target genes.
Library prep
Illumina 5-Base DNA Prep with Enrichment
A single assay for targeted detection of five DNA bases (A, T, G, C, and 5mC), providing dual insights into genomic variants and methylation events.
Sequencing
These mid-throughput sequencing systems offer expansive application breadth, operational simplicity, high flexibility and scalability, and proven performance.
Perform powerful high-throughput sequencing studies. Match data output and time to results to project needs.
Advanced chemistry, optics, and informatics combine to deliver exceptional sequencing speed and data quality, outstanding throughput, and scalability.
Data analysis
Accurate, comprehensive, and efficient secondary analysis of next-generation sequencing (NGS) data.
A cloud-based multiomic analysis and interpretation software, enabling sample to insight workflow with visualizations, scalability, and secure data management.
Learn more about the genome and methylome
Decode DNA and methylation together with the Illumina 5-base solution
Watch an overview of the Illumina 5-base solution to see how this single-assay solution simplifies multiomic analysis by integrating methylation detection and variant calling into one streamlined workflow.
Watch an overview of the Illumina 5-base solution to see how this single-assay solution simplifies multiomic analysis by integrating methylation detection and variant calling into one streamlined workflow.
Testimonial
I am excited about a method that overcomes the loss of data caused by classical approaches. The lllumina 5-base solution converts 5mC to T, rather than converting unmethylated C. This approach preserves sequence diversity, yields significantly more usable reads, and enhances the signal, improving our ability to perform DNA methylation analysis.
Hear from experts
Multiomic innovation in action
Using the Illumina 5-base solution, Dr Daniel Wise introduces a transformative research approach to colorectal cancer detection through an innovative, noninvasive sampling method that integrates multiomic, epigenetic, and metagenomic insights.
Multiomic innovation in action
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Validation of Illumina 5-base sequencing with EpiSignTM
Evaluation of Illumina 5-base sequencing with EpiSign
Dr Bekim Sadikovic, Director of the Molecular Genetics Laboratory at London Health Sciences Centre Research Institute, shares insights from evaluation of the Illumina 5-base solution.
Speak to a specialist
Learn how the Illumina 5-base solution can replace two separate genetic and epigenetic sequencing assays, saving time and budgets.
Illumina 5-Base DNA Prep
Offers simultaneous discovery of genomic variants and methylation events across the whole genome.
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Illumina 5-Base DNA Prep with Enrichment
Targeted sequencing of specific genomic regions for deeper, cost-effective analysis.
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