COVIDSeq检测(研究版本)

这一高通量新一代测序(NGS)检测方法可检测SARS-CoV-2病毒的RNA。Read More...

 

Key Features and Benefits
  • Accurate: Detects 98 targets on SARS-CoV-2 for highly accurate detection
  • Comprehensive: Reports consensus sequence when +90 amplicons are detected
  • Scalable: Up to 3,072 samples per run on NovaSeq 6000 S4 and SP flow cells or up to 384 samples per run on NextSeq 500/550/550Dx (in Research Mode)
  • Flexible: Seamless end to end workflow with built in quality control features

Product Highlights

Illumina COVIDSeq检测(版本)是一种基于扩增子的NGS检测方法,包含旨在研究检测SARS-CoV-2病毒RNA的2019-nCoV引物,可用于临床研究应用。

快速、可扩展的SARS-CoV-2检测

Illumina COVIDSeq检测(研究版本)可根据不同样本数量的需求扩大或缩小规模。在NovaSeq 6000系统上使用两个SP或S4试剂盒,可在12小时内分别处理1536至3072个结果,使用NextSeq 500/550/550Dx(研究模式)HO试剂盒,可在12小时内处理384个结果。

工作流程和组件

工作流程包含病毒RNA提取、RNA-cDNA转化、PCR、文库制备、测序、分析和报告生成等步骤。主要组件包括高通量NovaSeq 6000系统或NextSeq 500/550/550Dx(研究模式)系统,配合Illumina COVIDSeq检测(研究版本)和BaseSpace Sequence Hub中用于快速分析的DRAGEN COVIDSeq检测流程(研究版本)或DRAGEN COVIDSeq检测应用程序(研究版本)。

Design and Quality Control

The Illumina COVIDSeq Test (RUO version) leverages a modified version of the validated, publicly available ARTIC multiplex PCR protocol, with 98 amplicons designed to amplify SARS-CoV-2 virus-specific sequences, combined with proven Illumina sequencing technology. COVIDSeq provides consensus sequence information (FASTA file) that can be uploaded into open software tools to assign lineage and annotate mutations. FASTA files can be easily uploaded to NCBI and/or GISAID App directly through BaseSpace Sequencing Hub for added convenience.

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规格

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