The AmpliSeq for Illumina Cancer Hotspot Panel v2 is a targeted resequencing assay for researching somatic mutations across the hotspot regions of 50 genes with known associations to cancer, as identified in the Catalogue of Somatic Mutations in Cancer (COSMIC)1 database. Key features include:
The Cancer HotSpot Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This panel enables highly sensitive variant detection within multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate. The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
仪器 | 推荐的样本数 | 读长 |
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iSeq 100 System | 每次运行16个样本(假设最小覆盖度为500倍) | 2 × 150 bp |
MiniSeq System | 每次运行的样本:中通量:32,高通量:96(假设最小覆盖度为500倍) | 2 × 150 bp |
MiSeq System | 每次运行的样本(按试剂盒版本):v2 nano:4,v2 micro:16,v2:60,v3:96(假设最小覆盖度为500倍) | 2 × 150 bp |
AmpliSeq for Illumina Cancer Hotspot Panel v2 | AmpliSeq for Illumina Focus Panel | AmpliSeq for Illumina Comprehensive Panel v3 | TruSight Tumor 15 |
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AmpliSeq for Illumina Oncology Panels on the iSeq 100 System
Application Note | PDF< 1 MB
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
Sample Identification with the AmpliSeq for Illumina Sample ID Panel Application Note
Application Note | HTML
Data Sheet | HTML | 5 versions
AmpliSeq for Illumina Cancer HotSpot Panel Gene List
product_file | EXCEL< 1 MB
AmpliSeq for Illumina Cancer HotSpot Panel v2 Consumables & Equipment Documentation
Illumina Adapter Sequences Document Documentation
Index Adapters Pooling Guide Documentation
AmpliSeq for Illumina Direct FFPE DNA Kit Reference Guide Documentation
AmpliSeq for Illumina Cancer Hotspot Panel v2 Support - Documentation & Literature
AmpliSeq for Illumina Cancer HotSpot Panel v2 Reference Guide Documentation
AmpliSeq for Illumina Cancer HotSpot Panel v2 Checklist Documentation
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