Illumina Stranded mRNA Prep

一个简单、可扩展、经济、快速的解决方案,只需25 ng标准(未降解)RNA,即可在一天之内对编码转录组实现快速分析。Read More...
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Library Prep

Illumina® Stranded mRNA Prep, Ligation (16 Samples)

20040532

Illumina® Stranded mRNA Prep, Ligation (96 Samples)

20040534


Index Adapters

IDT® for Illumina® RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples)

20040553

IDT® for Illumina® RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples)

20040554


Services

Illumina Stranded mRNA Library Prep Training

20044765

Product Highlights

Illumina Stranded mRNA Prep offers a streamlined RNA-Seq solution for clear and comprehensive analysis across the transcriptome. It offers extraordinary flexibility for input type and supports low input amounts, down to 25 ng total RNA. Illumina Stranded mRNA Prep enables precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as novel isoforms, gene fusions, and allele-specific expression.

Exceptional Performance

Illumina Stranded mRNA Prep is optimized to provide exceptional polyA capture efficiency and coverage uniformity. It minimizes the required sequencing depth for accurate, unbiased detection of the coding transcriptome. Illumina Stranded mRNA Prep is based on TruSeq ligation technology, which has been cited in over 9,926 publications since 2011.

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Flexibility for Many Study Designs

Illumina Stranded total RNA Prep supports a broad range of RNA inputs, from 25 ng to 1000 ng.*

Precise Measurement of Strand Orientation

This information enables detection of antisense transcription, enhances transcript annotation, and increases alignment efficiency.

Fast, Automation-friendly Workflow

Prepare libraries for sequencing in less than 7 hours. Illumina-qualified automation options are coming soon through our automation partners.

Sequencing Scalability

Multiplex up to 384 samples and sequence in a single run. (Index Sets A and B are available now; Sets C and D are coming soon.)

The Power of RNA-Seq

RNA-Seq is increasingly the platform of choice for thought leaders, providing a detailed snapshot of the transcriptome at a given point in time. It offers numerous advantages over quantitative PCR, including:

  • Hypothesis-free experimental design, requiring no previous knowledge of the transcriptome 
  • Higher discovery power to detect known and novel transcripts 
  • Higher throughput capability to quantify hundreds to thousands of regions in each assay 
  • Broader dynamic range, providing more accurate measurement of gene expression 
  • More data per assay, providing full sequence and variant information

*Not compatible with FFPE samples

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
NextSeq 550 System Mid Output: 5
High Output: 16
(based on 25M reads per sample)
2 × 75 bp
NextSeq 2000 System P2: 16
P3: 40
(based on 25M reads per sample)
2 × 75 bp
NovaSeq 6000 System SP: 32
S1: 64
S2: 164
S4: 384
(based on 25M reads per sample)
2 × 75 bp

产品比较

Illumina Stranded mRNA Prep TruSeq Stranded mRNA TruSeq RNA Library Prep Kit v2 Illumina RNA Prep with Enrichment Illumina Stranded Total RNA Prep with Ribo-Zero Plus
Automation Capability 液体处理机器人 液体处理机器人 液体处理机器人 液体处理机器人 液体处理机器人
Strand Specificity Stranded Stranded Non-Stranded Non-Stranded Stranded
Technology 测序 测序 测序 测序 测序
Variant Class Novel Transcripts, Transcript Variants, 单核苷酸多态性 (SNPs), 基因融合, 转录变异 Novel Transcripts, Transcript Variants, 单核苷酸多态性 (SNPs), 基因融合, 转录变异 Novel Transcripts, Transcript Variants, 单核苷酸多态性 (SNPs), 基因融合, 转录变异 Novel Transcripts, Transcript Variants, 单核苷酸多态性 (SNPs), 基因融合, 转录变异 Novel Transcripts, Transcript Variants, 单核苷酸多态性 (SNPs), 基因融合, 转录变异

Method-Specific Workflow Example

 

Supporting Data and Figures

 

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Illumina RNA Prep with Enrichment

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Illumina Stranded Total RNA Prep with Ribo-Zero Plus

使用多种样本快速制备文库,以极大的灵活性来研究编码和非编码转录组。