Illumina Stranded Total RNA Prep with Ribo-Zero Plus

Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with unparalleled study flexibility.Read More...
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Library Prep

Illumina® Stranded Total RNA Prep Ligation with Ribo-Zero Plus 16 Samples

20040525

Illumina® Stranded Total RNA Prep Ligation with Ribo-Zero Plus 96 Samples

20040529


Index Adapters

IDT® for Illumina® RNA UD Indexes Set A Ligation (96 indexes, 96 samples)

20040553

IDT® for Illumina® RNA UD Indexes Set B Ligation (96 indexes, 96 samples)

20040554

Product Highlights

Illumina Total RNA Prep with Ribo-Zero Plus provides exceptional performance for the analysis of coding and multiple forms of noncoding RNA. Gain high-confidence discovery of alternative transcripts, gene fusions, and allele-specific expression.

Exceptional Performance

Illumina Total RNA Prep with Ribo-Zero Plus is based on TruSeq ligation technology, which has been cited in over 9,926 publications since 2011. This ligation method results in high coverage uniformity, precise strand information, and robust and reliable library preparation even from degraded samples.

Flexibility for Many Study Designs

Illumina Total RNA Prep with Ribo-Zero Plus supports a broad range of RNA inputs, from 1 ng to 1000 ng*. It’s compatible with variable sample types, including formalin-fixed paraffin-embedded (FFPE) and other low-quality samples. The included Ribo-Zero Plus removes abundant RNA from multiple species, including human, mouse, rat, bacteria, and epidemiology samples.

Find Compatible Species

Fast, Automation-friendly Workflow

Prepare libraries in as little as 7 hours. Illumina-qualified automation methods will be available soon through our automation partners.

Sequencing Scalability

Multiplex up to 384 samples and sequence in a single run. (Index Sets A and B are available now; Sets C and D are coming soon.) The number of samples sequenced per run depends on the throughput capacity of the sequencer.

*This input range is applicable to high-quality RNA. We recommend 10 ng input for best results and for FFPE or degraded samples.

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
NextSeq 550 System Mid output: 2
High output: 8
(based on 50M reads per sample)
2 × 75 bp
NextSeq 2000 System Whole Transcriptome Analysis:
8 (P2)-50 (P3) samples per run (based on 50M reads per sample)
2 × 75 bp
NovaSeq 6000 System Whole Transcriptome Analysis:
SP: 16
S1: 32
S2: 82
S4: 384
samples per run (based on 50M reads per sample)
2 × 75 bp

产品比较

Illumina Stranded Total RNA Prep with Ribo-Zero Plus TruSeq Stranded Total RNA TruSeq Stranded Total RNA with Ribo-Zero Globin
Automation Capability Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots
Method Whole-Transcriptome Sequencing Whole-Transcriptome Sequencing Whole-Transcriptome Sequencing
Specialized Sample Types FFPE Tissue, Low-Input Samples FFPE Tissue
System Compatibility HiSeq 2500 , HiSeq 3000 , HiSeq 4000 , NextSeq 2000 , NextSeq 500 , NextSeq 550 , NovaSeq 6000 HiSeq 1000, HiSeq 1500, HiSeq 2000, HiSeq 2500 , HiSeq 3000 , HiSeq 4000 , NextSeq 500 , NextSeq 550 , NovaSeq 6000 HiSeq 1000, HiSeq 1500, HiSeq 2000, HiSeq 2500 , HiSeq 3000 , HiSeq 4000 , NextSeq 500 , NextSeq 550 , NovaSeq 6000
Technology Sequencing Sequencing Sequencing
Variant Class Gene Fusions, Novel Transcripts, Single Nucleotide Polymorphisms (SNPs), Transcript Variants Gene Fusions, Novel Transcripts, Single Nucleotide Polymorphisms (SNPs), Transcript Variants Gene Fusions, Novel Transcripts, Single Nucleotide Polymorphisms (SNPs), Transcript Variants

Method-Specific Workflow Example

 

Supporting Data and Figures

 

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