TruSeq Stranded Total RNA
可靠的、扩展自如的全转录组分析（RNA-Seq）解决方案适用于各种物种与样本类型，包括人类、小鼠与福尔马林固定石蜡包埋（FFPE）样本。Read More...
以前名为TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat（货号RS-122-2201、RS-122-2202和RS-122-2203）和TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold（货号RS-122-2301、RS-122-2302和RS-122-2303）的产品现分别更名为TruSeq Stranded Total RNA Human/Mouse/Rat和TruSeq Stranded Total RNA Gold。该产品的配置已更改。在新的配置中，文库制备试剂和标签接头均可单独购买。可单独购买的组件列表可在“选择产品”部分中找到。
TruSeq Stranded Total RNA提供了清晰的转录组视图，为总RNA测序分析提供了经济高效的一体化可扩展解决方案。
TruSeq Stranded Total RNA可兼容包括低质量DNA/RNA和福尔马林固定石蜡包埋（FFPE）样本在内的多种样本，结合了TruSeq RNA技术与Ribo-Zero核糖体RNA去除方法的所有优点。该产品可分析编码和多种形式的非编码RNA，能够精确测量链方向，实现均匀覆盖，并发现高度可信的替代转录本、基因融合和等位基因特异性表达等特征。
TruSeq Stranded Total RNA专为人类、小鼠和大鼠测序而设计，但也可能适用于各类真核生物物种。
TruSeq Stranded Total RNA有多种版本，可满足特定研究设计需要：
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| NextSeq 550 System | RNA Profiling: 6-20 samples per run (based on 20 million reads per
sample) Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample) |
2 x 75 bp |
| HiSeq 2500 System | RNA Profiling: 30-200 samples per run (dual flow cell; based on 20
million reads per sample) Transcriptome Analysis: 12-80 samples per run (dual flow cell; based on 50 million reads per sample) |
2 x 75 bp |
| NovaSeq 6000 System | RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4:
768. Limited by index combinations (dual). Based on 20 million reads. Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads. |
≤ 2 × 100 bp |
| TruSeq Stranded Total RNA | TruSeq Stranded Total RNA with Ribo-Zero Globin | |
|---|---|---|
| Assay Time | 11.5 hours | 11.5 hours |
| Automation Capability | 液体处理机器人 | 液体处理机器人 |
| Content Specifications | 捕获编码RNA以及多种形式的非编码RNA。 | 捕获编码RNA以及多种形式的非编码RNA。 |
| Description | For whole-transcriptome sequencing studies. The Total RNA version depletes cytoplasmic rRNA while the Total RNA Gold version depletes cytoplasmic and mitochondrial rRNA. | 用于血液来源RNA的全转录组测序研究。去除细胞质rRNA、线粒体rRNA和珠蛋白mRNA。 |
| Hands-On Time | 5.5 hours | 5.5 hours |
| Input Quantity | 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization. | 0.1–1 ug血液来源的高质量纯化总RNA |
| Mechanism of Action | 基于微珠的rRNA去除、cDNA合成和PCR | 基于微珠的rRNA去除、cDNA合成和PCR |
| Method | 全转录组测序 | 全转录组测序 |
| Multiplexing | 低通量试剂盒：混合最多12个样本。或混合24个样本（组A和B一起）, 高通量试剂盒：使用组合双标签或唯一双标签序列制备96个带标签的样本。 | 高通量试剂盒：使用组合双标签或唯一双标签序列制备96个带标签的样本。, 低通量试剂盒：混合最多12个样本。或混合24个样本（组A和B一起） |
| Specialized Sample Types | FFPE Tissue | Blood |
| Species Category | 人类, 大鼠, 小鼠 | 人类, 大鼠, 小鼠 |
| Species Details | rRNA Removal: Depletes human, mouse, and rat rRNA | rRNA Removal: Depletes human, mouse, and rat rRNA (cytoplasmic and mitochondrial) and globin mRNA |
| Technology | 测序 | 测序 |
BaseSpace Sequence Hub helps the Biotechnology Resource Center Sequencing Core (BRC-Seq) at the University of British Columbia deliver high-quality sequencing data and additional value to its customers.
Read MoreRobust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
Application Note | HTML
Data Sheet | PDF | 5 versions
TruSeq Stranded Total RNA With Illumina Ribo-Zero Plus rRNA Depletion Reference Guide Documentation
TruSeq Stranded Total RNA Documentation
TruSeq Stranded Total RNA Checklist Documentation
TruSeq Stranded Total RNA Consumables & Equipment Documentation
TruSeq Stranded Total RNA Reference Guide Documentation
自定义协议选择器
Generates customized, end-to-end instructions
Determine the best library prep kit or array for your needs based on your starting material and method of interest.
Access Tool
Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes ribosomal RNA and globin mRNA in a single step.
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