TruSeq Stranded Total RNA
可靠的、扩展自如的全转录组分析（RNA-Seq）解决方案适用于各种物种与样本类型，包括人类、小鼠与福尔马林固定石蜡包埋（FFPE）样本。Read More...
以前名为TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat（货号RS-122-2201、RS-122-2202和RS-122-2203）和TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold（货号RS-122-2301、RS-122-2302和RS-122-2303）的产品现分别更名为TruSeq Stranded Total RNA Human/Mouse/Rat和TruSeq Stranded Total RNA Gold。该产品的配置已更改。在新的配置中，文库制备试剂和标签接头均可单独购买。可单独购买的组件列表可在“选择产品”部分中找到。
TruSeq Stranded Total RNA提供了清晰的转录组视图，为总RNA测序分析提供了经济高效的一体化可扩展解决方案。
TruSeq Stranded Total RNA可兼容包括低质量DNA/RNA和福尔马林固定石蜡包埋（FFPE）样本在内的多种样本，结合了TruSeq RNA技术与Ribo-Zero核糖体RNA去除方法的所有优点。该产品可分析编码和多种形式的非编码RNA，能够精确测量链方向，实现均匀覆盖，并发现高度可信的替代转录本、基因融合和等位基因特异性表达等特征。
TruSeq Stranded Total RNA专为人类、小鼠和大鼠测序而设计，但也可能适用于各类真核生物物种。
TruSeq Stranded Total RNA有多种版本，可满足特定研究设计需要：
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| NextSeq 550 System | RNA Profiling: 6-20 samples per run (based on 20 million reads per
sample) Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample) |
2 x 75 bp |
| HiSeq 2500 System | RNA Profiling: 30-200 samples per run (dual flow cell; based on 20
million reads per sample) Transcriptome Analysis: 12-80 samples per run (dual flow cell; based on 50 million reads per sample) |
2 x 75 bp |
| NovaSeq 6000 System | RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4:
768. Limited by index combinations (dual). Based on 20 million reads. Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads. |
≤ 2 × 100 bp |
| TruSeq Stranded Total RNA | TruSeq Stranded Total RNA with Ribo-Zero Globin | |
|---|---|---|
| Automation Capability | 液体处理机器人 | 液体处理机器人 |
| Method | Whole-Transcriptome Sequencing | Whole-Transcriptome Sequencing |
| Specialized Sample Types | FFPE Tissue | Blood |
| Species Category | Mouse, Rat, 人类 | Mouse, Rat, 人类 |
| Technology | 测序 | 测序 |
Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
Application Note | HTML
Data Sheet | PDF | 5 versions
TruSeq Stranded Total RNA With Illumina Ribo-Zero Plus rRNA Depletion Reference Guide Documentation
TruSeq Stranded Total RNA Documentation
TruSeq Stranded Total RNA Checklist Documentation
TruSeq Stranded Total RNA Consumables & Equipment Documentation
TruSeq Stranded Total RNA Reference Guide Documentation
自定义协议选择器
Generates customized, end-to-end instructions
Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes ribosomal RNA and globin mRNA in a single step.
有限标签使用许可：此产品及其使用受Max Planck Gesellschaft所拥有且独家授权给New England Biolabs, Inc.并再授权给Illumina, Inc.的一个或多个已公告和/或待批准的美国和外国专利申请的约束。向Illumina, Inc.、其关联机构或其授权经销商和分销商购买此产品，即向买方转让使用买方所购数量产品和产品组件的权利（不论买方是学术性还是盈利性实体），该权利不可转让。购买本产品不会获得上述专利或专利申请权利请求项下的产品生产许可。买方不得向第三方出售或以其他方式转让此产品及其组件，亦不得以其他方式将此产品用于下商业用途：（1）在生产中使用此产品或其组件；或（2）出于治疗或预防疾病的目的，在人或动物身上使用此产品或其组件。
