With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA.
Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatible with all Illumina sequencers.
The IDT® for Illumina®-Nextera™ DNA UD Indexes Sets A, B, C, and D Indexes offer up to 384 unique dual indexing that enables accurate assignment of reads and efficient use of the flow cell. These unique dual index codes use 10 base pair codes. This change in base pair index codes require adjustments to the sequencing run setup.
Find automation vendors with robotic systems compatible with this kit
This product is available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.
For high-throughput research, bead-based normalization can save time and resources by providing an accelerated path from DNA to data. This technical note provides guidelines and data comparing the two normalization methods to help you decide which option to use.
Read Tech Note仪器 | 推荐的样本数 | 读长 |
---|---|---|
MiSeq System | Up to 384 samples per run | Up to 2 x 300 bp |
NextSeq 550 System | Up to 384 samples per run | Up to 2 x 150 bp |
Nextera XT DNA Library Preparation Kit | Illumina DNA Prep | |
---|---|---|
Method | 16s rRNA Sequencing, Amplicon Sequencing , De Novo Sequencing , Shotgun Sequencing , Whole-Genome Sequencing | Amplicon Sequencing , De Novo Sequencing , Shotgun Sequencing , Whole-Genome Sequencing |
Specialized Sample Types | Low-Input Samples, Single Cells | Blood, Not FFPE-Validated, Saliva |
Nextera Library Validation and Cluster Density Optimization
Technical Note | PDF< 1 MB
Technical Note | PDF< 1 MB
Nextera XT Library Prep: Tips and Troubleshooting
Technical Note | PDF< 1 MB
Sequencing the Rapidly Evolving Influenza A Virus on the MiSeq System
Application Note | PDF< 1 MB
Nextera XT DNA Library Preparation Kit
Data Sheet | PDF< 1 MB
Nextera XT DNA Library Prep Kit Documentation
Nextera XT DNA Library Prep Kit Consumables & Equipment List Documentation
Index Adapters Pooling Guide Documentation
Nextera XT DNA Library Prep Kit Checklist Documentation
Illumina Experiment Manager User Guide Documentation
Nextera XT DNA Library Prep Kit Reference Guide Documentation
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