Nextera XT DNA Library Preparation Kit

支持低DNA起始量,仅需90分钟,即可为小型基因组、PCR扩增子、质粒或cDNA制备好测序文库。Read More...
选择产品
What products do I need?
Library Prep

Nextera XT DNA Library Preparation Kit (24 samples)

FC-131-1024

Nextera XT DNA Library Preparation Kit (96 samples)

FC-131-1096


Illumina Advantage Products

TG Nextera® XT DNA Sample Preparation Kit (96 Samples)

TG-131-1096

TG Nextera® XT Index Kit v2 Set A (96 Indices, 384 Samples)

TG-131-2001

TG Nextera® XT Index Kit v2 Set B (96 Indices, 384 Samples)

TG-131-2002


Index Adapters

IDT® for Illumina Nextera DNA Unique Dual Indexes Set A (96 Indexes, 96 Samples)

20027213

IDT® for Illumina Nextera DNA Unique Dual Indexes Set B (96 Indexes, 96 Samples)

20027214

IDT® for Illumina Nextera DNA Unique Dual Indexes Set C (96 Indexes, 96 Samples)

20027215

IDT® for Illumina Nextera DNA Unique Dual Indexes Set D (96 Indexes, 96 Samples)

20027216

Nextera XT Index Kit v2 Set A (96 indexes, 384 samples)

FC-131-2001

Nextera XT Index Kit v2 Set B (96 indexes, 384 samples)

FC-131-2002

Nextera XT Index Kit v2 Set C (96 indexes, 384 samples)

FC-131-2003

Nextera XT Index Kit v2 Set D (96 indexes, 384 samples)

FC-131-2004

Nextera XT Index Kit (24 indexes, 96 samples)

FC-131-1001

配件产品
What accessories do I need?

Illumina Free Adapter Blocking Reagent (12 reactions)

20024144

Illumina Free Adapter Blocking Reagent (48 reactions)

20024145

Product Highlights

Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids
  • Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time
  • Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.
  • Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications
  • Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatible with all Illumina sequencers.

The IDT® for Illumina®-Nextera™ DNA UD Indexes Sets A, B, C, and D Indexes offer up to 384 unique dual indexing that enables accurate assignment of reads and efficient use of the flow cell.  These unique dual index codes use 10 base pair codes. This change in base pair index codes require adjustments to the sequencing run setup.

Find automation vendors with robotic systems compatible with this kit

This product is available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

When to Use Standard vs. Bead-Based Normalization

For high-throughput research, bead-based normalization can save time and resources by providing an accelerated path from DNA to data. This technical note provides guidelines and data comparing the two normalization methods to help you decide which option to use.

Read Tech Note
When to Use Standard vs. Bead-Based Normalization

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
MiSeq System Up to 384 samples per run Up to 2 x 300 bp
NextSeq 550 System Up to 384 samples per run Up to 2 x 150 bp

产品比较

Method-Specific Workflow Example

 

Customer Stories

绘制神经元多样性:小鼠初级视觉皮质中神经员标识的分子分析

通过 MiSeq和HiSeq系统执行的单细胞mRNA-Seq,艾伦研究所(Allen Institute)研究人员可分类V1神经元细胞。

了解更多
新一代测序帮助研究人员抗击埃博拉病毒

通过采用HiSeq and MiSeq系统进行基因组学研究,研究人员可跟踪埃博拉爆发并了解病毒快速突变的影响。

了解更多

Supporting Data and Figures

 

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