Nextera XT DNA文库制备试剂盒

支持低DNA起始量,仅需90分钟,即可为小型基因组、PCR扩增子、质粒或cDNA制备好测序文库。Read More...
选择产品
What products do I need?
Library Prep

Nextera XT DNA Library Preparation Kit (24 samples)

FC-131-1024

Nextera XT DNA Library Preparation Kit (96 samples)

FC-131-1096


Illumina Advantage Products

TG Nextera® XT DNA Sample Preparation Kit (96 Samples)

TG-131-1096

TG Nextera® XT Index Kit v2 Set A (96 Indices, 384 Samples)

TG-131-2001

TG Nextera® XT Index Kit v2 Set B (96 Indices, 384 Samples)

TG-131-2002


Index Adapters

IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

IDT® for Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20027214

IDT® for Illumina Nextera DNA Unique Dual Indexes Set C (96 Indexes, 96 Samples)

20027215

IDT® for Illumina Nextera DNA Unique Dual Indexes Set D (96 Indexes, 96 Samples)

20027216

Nextera XT Index Kit v2 Set A (96 indexes, 384 samples)

FC-131-2001

Nextera XT Index Kit v2 Set B (96 indexes, 384 samples)

FC-131-2002

Nextera XT Index Kit v2 Set C (96 indexes, 384 samples)

FC-131-2003

Nextera XT Index Kit v2 Set D (96 indexes, 384 samples)

FC-131-2004

Nextera XT Index Kit (24 indexes, 96 samples)

FC-131-1001

配件产品
What accessories do I need?

Illumina Purification Bead, 100mL

20060057

Illumina Purification Bead, 400mL

20060058

Illumina® Free Adapter Blocking Reagent (12 Reactions)

20024144

Illumina® Free Adapter Blocking Reagent (48 Reactions)

20024145

产品特色

快速文库准备,优化小基因组,PCR扩增物和质粒
  • 快速文库制备 –只需 15 分钟的动手时间,只需 90 分钟即可完成文库制备工作
  • 快速出结果 – 用我们的台式测序仪在8小时内将DNA转化为数据。
  • 推荐用于小基因组、PCR扩增物和质粒 – 一款适用于多种应用的文库制备工具包
  • 创新的样本标准化 – 无需在样本合并和测序之前进行文库量化

借助 Nextera 技术,DNA 在单管酶促反应中同时被片段化并用测序接头进行标记。 Nextera XT 支持仅 1 ng 的超低 DNA 输入量。它支持广泛的输入样本,包括小基因组、大于 300 bp 的 PCR 扩增子、质粒、微生物基因组、串联扩增子和双链 cDNA。

了解有关该技术的更多信息

Flexible Multiplexing

每个 Nextera XT 库可复用多达 384 个样本,可用于需要更高通量的项目。此外,该试剂盒包括创新的基于微珠的样品标准化,无需在合并和测序之前对文库进行量化。使用 Nextera XT 试剂盒制备的文库与所有 Illumina 测序仪兼容。

用于 Illumina-Nextera DNA UD 索引集 A、B、C 和 D 索引的 IDT 提供多达 384 个独特的双索引,允许准确分配读取和高效使用流动槽。这些独特的双索引代码使用 10 bp 代码。碱基对索引代码的这种变化需要对测序运行设置进行调整。

查找与此套件兼容的机器人系统

该产品也可作为 Illumina Advantage (TG) 产品。Illumina Advantage大规模测序产品具有特定批次出货和测试、延长货架寿命和先进的变更通知,以提高实验室效率。

When to Use Standard vs. Bead-Based Normalization

For high-throughput research, bead-based normalization can save time and resources by providing an accelerated path from DNA to data. This technical note provides guidelines and data comparing the two normalization methods to help you decide which option to use.

Read Tech Note
When to Use Standard vs. Bead-Based Normalization

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
MiSeq System Up to 384 samples per run Up to 2 x 300 bp
NextSeq 550 System Up to 384 samples per run Up to 2 x 150 bp

产品比较

Nextera XT DNA文库制备试剂盒 Illumina DNA Prep
Assay Time ~5.5 hours from DNA extraction to normalized library. (Library prep time: ~90 minutes). ~3-4 hours (from DNA extraction to normalized library)
Description Fast library prep optimized for research on small genomes, PCR amplicons, and plasmids. A fast, flexible workflow for a wide range of research applications and sample types, from human to microbial whole-genome sequencing and more.
Hands-On Time 15 minutes 1-1.5 hours
Input Quantity 1 ng DNA Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. (For blood and saliva, see the reference guide).
Mechanism of Action Enzymatic fragmentation 连接磁珠的转座酶
Method 16s rRNA Sequencing, Amplicon Sequencing , De Novo Sequencing , Shotgun Sequencing , Whole-Genome Sequencing Amplicon Sequencing , De Novo Sequencing , Shotgun Sequencing , Whole-Genome Sequencing
Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together. Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations
Specialized Sample Types Low-Input Samples, Single Cells, 不兼容FFPE Blood, Not FFPE-Validated, Saliva
Species Details Compatible with any species Compatible with any species
Target Insert Size 300 bp–1.5 kb ~350bp

Method-Specific Workflow Example

 

Customer Stories

Mapping Neural Diversity: A Molecular Analysis of Neuronal Identity in Mouse Primary Visual Cortex

Single-cell mRNA-Seq with the MiSeq and HiSeq Systems is enabling Allen Institute researchers to classify V1 neural cells.

Read More
Next-Generation Sequencing Aids Researchers in the Fight Against the Ebola Virus

Genomic studies with the HiSeq and MiSeq Systems are enabling researchers to track Ebola outbreaks and understand the impact of the virus’s rapid mutation rate.

Read More

Supporting Data and Figures

 

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