主要应用和方法
获得最小生物的详细基因组图谱。小型基因组测序可对微生物或病毒基因组进行全面分析，有助于开展公共卫生、流行病学和疾病研究。每次MiSeq运行能对多达24个小型基因组进行测序。
查看其他研究人员如何使用MiSeq系统的小型基因组测序功能来进行微生物基因组研究：
Library Prep
90 mins/15 mins hands-on
Nextera XT Library Prep Kit
Prepare sequencing libraries for small genomes, PCR amplicons, and plasmids in less than 90 min, with a low DNA input requirement.
Nextera DNA Flex Library Prep Kit
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
Sequence
56 hours/15 mins hands-on
MiSeq Reagent Kit v3 600 cycles
Optimized chemistry to increase cluster density and read length, and improve sequencing quality scores, compared to earlier kit versions.
Analyze
3 hours
SPAdes Genome Assembler
Open source tool for de novo sequencing, designed to assemble small genomes from MDA single-cell and standard bacterial data sets.
Browse sample data in Basespace Sequence Hub (login required):
MiSeq Small Genome Data
靶向重测序专注于对基因子集或感兴趣研究区域的基因组的分析，节省时间与花费。扩增子测序（PCR扩增子的超深度测序）能够在单次分析中对多达数百个目标基因组区域进行经济有效地分析。在单次MiSeq运行中可测序多达96个样本和1536个扩增子或更多。
查看其他研究人员如何使用MiSeq系统进行扩增子测序：
Assay Design & Library Prep
5-7 hours
AmpliSeq for Illumina Sequencing Solution
A highly multiplexed polymerase chain reaction (PCR)-based workflow for use with targets ranging from a few to hundreds of genes in a single run.
DesignStudio Software
Sequence
17-32 hours
MiSeq Reagent Kit v2 (300 cycles)
MiSeq sequencing reagents in pre-filled, ready-to-use cartridges. Micro and nano formats are available for low output applications.
Data Analysis
3 hours
Local Run Manager
An on-premises software solution for creating sequencing runs, monitoring run status, and analyzing data.
BaseSpace Sequence Hub
The Illumina genomics cloud computing environment for NGS data analysis and management.
BaseSpace Variant Interpreter
Enables researchers to rapidly identify biologically significant variants from human genomic data.
16S rRNA（核糖体RNA）测序法无需培养，可用于鉴定和比较那些难以研究的，来自复杂微生物组或环境的细菌。 我们演示的16S rRNA测序方案可以帮助您消除实验中的相关猜测。多重分析功能可让您在一次MiSeq运行中处理多达96个样本。
查看其他研究人员如何使用MiSeq系统来推动宏基因组学研究：
Library Prep
3 hours/30 mins hands-on
Nextera XT Index Kit v2
Nextera XT index kits allow for up to 384 uniquely indexed samples to be pooled and sequenced on a single sequencing run.
Sequence
56 hours/15 mins hands-on
MiSeq Reagent Kit v3 (600 cycles)
Optimized chemistry to increase cluster density and read length, and improve sequencing quality scores, compared to earlier kit versions.
Analyze
3 hours
16S Metagenomics BaseSpace App
Performs taxonomic classification of 16S rRNA targeted amplicon reads using an Illumina-curated version of the GreenGenes taxonomic database.
Browse sample data in BaseSpace Sequence Hub (login required):
16S Metagenomic Sequencing Run
16S Metagenomic Sequencing Project
每个样本的估算成本：18美元*
*在2016年使用MiSeq系统开展16S rRNA测序的每个样本的估算成本，基于96个样本、2 x 300 bp的读长，使用Nextera XT标签引物和MiSeq Reagent Kit v3（600个循环）
更多应用和方法
使用靶向RNA-Seq进行基因表达分析
靶向RNA测序（RNA-seq）主要关注特定的感兴趣的转录本，可用于分析基因表达和鉴定融合基因。
应用说明
适用于MiSeq系统的TruSeq Targeted RNA Expression解决方案
研究人员访谈
靶向基因panel
靶向基因测序panel包含定义好的探针组，专注于感兴趣的特定基因。预设计的panel和定制panel均可供您选用。
研究人员访谈
miRNA和小RNA分析
分离并测序小RNA，例如microRNA，研究非编码RNA在基因沉默和转录后调控中的作用。
使用染色质免疫沉淀测序（ChIP-Seq）进行DNA-蛋白质互作分析
将染色质免疫沉淀（ChIP）方法与测序结合在一起，ChIP-Seq是基因调控全基因组研究的强大方法。
研究人员访谈
方法指南
得到您需要的信息——包括用于基因组、转录组或表观基因组研究文库制备的BeadChip芯片、测序仪选择、分析及支持——集于一处。通过我们特别为研究应用设计的综合指南为您的实验室选择合适的工具。
获取指南