This sequencing research panel targets variants associated with myeloid malignancies such as AML, MDS, MPN, CML, CMML, and JMML.
Assay time
Hands-on time
Input quantity
The TruSight Myeloid Sequencing Panel uses expert-defined content and proven next-generation sequencing (NGS) technology to identify somatic variants in myeloid malignancies.
The panel content was designed by a consortium of recognized experts in blood cancer disorders and targets genes frequently mutated in:
The TruSight Myeloid Sequencing Panel covers 15 full genes (exons only) and 39 additional genes where oncogenic hotspots are covered, providing a comprehensive assessment of the key genes known to be involved in myeloid malignancies in a single test. The result is an accurate, cost-effective solution that enables researchers to profile liquid tumors. View the gene list in the "Figures" section.
TruSight Myeloid libraries are ideally suited to run on a desktop sequencer and perform alignment and variant calling with the MiSeq Reporter or Local Run Manager Amplicon workflow with Somatic Variant Caller. Filtering and annotation can then be performed using variant analysis software.
| Assay time | 8 hours |
|---|---|
| Cancer type | Hematologic |
| Content specifications | Targets 54 genes associated with myeloid malignancies. Probes amplify 568 amplicons from tumor suppressor genes and mutational hotspots. |
| Description | For somatic mutation detection studies in myeloid malignancies. |
| Hands-on time | 3 hr |
| Input quantity | 50 ng |
| Instruments | MiSeq System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System |
| Method | Targeted DNA sequencing, Amplicon sequencing |
| Multiplexing | Up to 96-plex |
| Nucleic acid type | DNA |
| Specialized sample types | Not FFPE-compatible, Not FFPE-compatible |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Somatic variants, Insertions-deletions (indels) |
Advanced chemistry, optics, and informatics combine to deliver exceptional sequencing speed and data quality, outstanding throughput, and scalability.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
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