This sequencing research panel targets variants associated with myeloid malignancies such as AML, MDS, MPN, CML, CMML, and JMML.
Assay time
Hands-on time
Input quantity
The TruSight Myeloid Sequencing Panel uses expert-defined content and proven next-generation sequencing (NGS) technology to identify somatic variants in myeloid malignancies.
The panel content was designed by a consortium of recognized experts in blood cancer disorders and targets genes frequently mutated in:
The TruSight Myeloid Sequencing Panel covers 15 full genes (exons only) and 39 additional genes where oncogenic hotspots are covered, providing a comprehensive assessment of the key genes known to be involved in myeloid malignancies in a single test. The result is an accurate, cost-effective solution that enables researchers to profile liquid tumors. View the gene list in the "Figures" section.
TruSight Myeloid libraries are ideally suited to run on a desktop sequencer and perform alignment and variant calling with the MiSeq Reporter or Local Run Manager Amplicon workflow with Somatic Variant Caller. Filtering and annotation can then be performed using variant analysis software.
| Assay time | 8 hours |
|---|---|
| Cancer type | Hematologic |
| Content specifications | Targets 54 genes associated with myeloid malignancies. Probes amplify 568 amplicons from tumor suppressor genes and mutational hotspots. |
| Description | For somatic mutation detection studies in myeloid malignancies. |
| Hands-on time | 3 hr |
| Input quantity | 50 ng |
| Instruments | MiSeq System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System |
| Method | Targeted DNA sequencing, Amplicon sequencing |
| Multiplexing | Up to 96-plex |
| Nucleic acid type | DNA |
| Specialized sample types | Not FFPE-compatible, Not FFPE-compatible |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Somatic variants, Insertions-deletions (indels) |
Each TruSight Myeloid library preparation kit (96 samples) requires the following accessory kits:
TruSight Myeloid Sequencing Panel
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| MiniSeq System | Samples per run: mid output: 2-3, high output: 8 (based on 95% amplicons at > 500x coverage) |
2 × 150 bp (max recommended) |
| MiSeq System | 8 samples per run with v3 reagents (based on 95% amplicons at > 500x coverage) |
2 × 150 bp (max recommended) |
| NextSeq 550 System | Samples per run: mid output: 48, high output: 96 (based on 95% amplicons at > 500x coverage) |
2 × 150 bp (max recommended) |
Pathology and clinical cancer research
Our clinical cancer research solutions deliver accurate genomic information, and enable labs to analyze multiple genes in a single test.
Ultra-deep sequencing of PCR amplicons enables analysis of specific genomic regions of interest. Learn more about amplicon sequencing and find comprehensive solutions.
NGS-based cancer sequencing methods provide more information in less time compared to single-gene and array-based approaches.
| TruSight Myeloid Sequencing Panel | AmpliSeq for Illumina Myeloid Panel | AmpliSeq for Illumina Cancer Hotspot Panel v2 | |
|---|---|---|---|
| Assay time | 8 hours | 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) |
| Cancer type | Hematologic | Hematologic | Pan-cancer, Solid tumor |
| Content specifications | Targets 54 genes associated with myeloid malignancies. Probes amplify 568 amplicons from tumor suppressor genes and mutational hotspots. | Targets biomarkers across 69 genes (40 key DNA target genes, 29 RNA fusion driver genes, and 5 gene expression levels) | Hotspot regions of 50 genes with known associations to cancer |
| Description | For somatic mutation detection studies in myeloid malignancies. | Targeted panel to study 40 DNA genes, 29 RNA fusion driver genes, and 5 gene expression levels associated with myeloid cancers. | Somatic analysis research into hotspot regions of 50 cancer-related genes. |
| Hands-on time | 3 hr | < 1.5 hr | < 1.5 hr |
| Input quantity | 50 ng |
20 ng high-quality DNA; 10 ng high-quality RNA (10 ng recommended per pool) |
1–100 ng (10 ng recommended per pool) |
| Instruments | MiSeq System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System | MiSeq System, MiniSeq System | MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System |
| Method | Targeted DNA sequencing, Amplicon sequencing | Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing | Targeted DNA sequencing, Amplicon sequencing |
| Multiplexing | Up to 96-plex | Up to 96-plex | 96 dual index combinations |
| Nucleic acid type | DNA | DNA, RNA | DNA |
| Specialized sample types | Not FFPE-compatible, Not FFPE-compatible | Blood, Not FFPE-compatible, Bone marrow | FFPE tissue |
| Species category | Human | Human | Human |
| Technology | Sequencing | Sequencing | Sequencing |
| Variant class | Somatic variants, Insertions-deletions (indels) | Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels) | Single nucleotide polymorphisms (SNPs), Somatic variants, Insertions-deletions (indels) |
| ABL1 | CEBPA | HRAS | MYD88 | SF3B1 |
| ASXL1 | CSF3R | IDH1 | NOTCH1 | SMC1A |
| ATRX | CUX1 | IDH2 | NPM1 | SMC3 |
| BCOR | DNMT3A | IKZF1 | NRAS | SRSF2 |
| BCORL1 | ETV6/TEL | JAK2 | PDGFRA | STAG2 |
| BRAF | EZH2 | JAK3 | PHF6 | TET2 |
| CALR | FBXW7 | KDM6A | PTEN | TP53 |
| CBL | FLT3 | KIT | PTPN11 | U2AF1 |
| CBLB | GATA1 | KRAS | RAD21 | WT1 |
| CBLC | GATA2 | MLL | RUNX1 | ZRSR2 |
| CDKN2A | GNAS | MPL | SETBP1 |
TruSight Myeloid Sequencing Panel (96 samples)
FC-130-1010
TruSeq Custom Amplicon-based library preparation reagents for 54 genes (either full genes or targeted regions) associated with myeloid malignancies. Includes library preparation reagents and TruSight Myeloid oligos sufficient for 96 samples.
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TruSeq Custom Amplicon Index Kit (96 indexes, 384 samples)
FC-130-1003
Each TruSeq Custom Amplicon Index Kit includes 96 unique indexes for preparation of up to 384 samples.
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TruSeq Custom Amplicon Filter Plate (1 plate)
FC-130-1006
Includes a single replacement filter plate for use with the TruSeq Custom Amplicon assay.
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TruSeq Index Plate Fixture & Collar Kit (2 each)
FC-130-1007
Assists in correctly arranging index primers for PCR amplification step. Includes two (2) TruSeq Index Plate Fixtures and two (2) Plate Collars.
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