TruSeq ChIP Library Preparation Kit

Simple, cost-effective chromatin immunoprecipitation sequencing (ChIP-Seq) DNA library preparation, with master mixes and robust multiplex capabilities.

Overview

TruSeq ChIP Libary Preparation Kits provide a simple, cost-effective solution for generating chromatin immunoprecipitation sequencing (ChIP-Seq) libraries from ChIP-derived DNA. ChIP-Seq leverages next-generation sequencing (NGS) to quickly and efficiently determine the distribution and abundance of DNA-bound protein targets of interest across the genome. 

This method has become one of the most widely used NGS applications, enabling researchers to reliably and simultaneously identify binding sites of a broad range of targets across the entire genome, with high resolution and without constraints.

TruSeq ChIP Library Preparation Kits are compatible with all Illumina sequencers. Offering the proven data quality and ease of use of TruSeq DNA sequencing, they provide a streamlined ChIP-Seq library preparation workflow that leverages reagent master mixes to minimize pipetting and reduce total assay time.

The kits have a low DNA input requirement and are compatible with a range of sample sources. In addition, they offer more robust multiplex sequencing with 24 unique indexes, allowing researchers to optimize the distribution of sequencing output across samples based on read depth requirements.


Specifications

Applications

Example workflow


Related applications and methods

Resources

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Epigenomics of Bird Brain: Modeling Human Speech Circuitry from Songs

Singing in songbirds is a model used to study speech production in humans. Sequencing is helping us closely understand this complex circuitry.Researchers identified that gene regulation is specific to each region of the bird brain involved in song production. It is a combination of region-specific transcription factors, their binding motifs, and histone regulated gene activation. 

TruSeq ChIP Library Preparation Kit - Set A (12 indexes, 48 rxns)

IP-202-1012

Kitted reagents for library preparation of 48 ChIP DNA samples for stranded single read, paired end, or multiplexed sequencing. Each kit contains 12 of 24 unique indexes. Each index is sufficient for 8 individual samples (Set A indexes 2, 4, 5, 6, 7, 12, 13, 14, 15, 16, 18, 19).

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TruSeq ChIP Sample Preparation Kit - Set B (12 indexes, 48 rxns)

IP-202-1024

Kitted reagents for library preparation of 48 ChIP DNA samples for stranded single read, paired end, or multiplexed sequencing. Each kit contains 12 of 24 unique indexes. Each index sufficient for 8 individual samples (Set B indexes 1, 3, 8, 9, 10, 11, 20, 21, 22, 23, 25, 27).

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