Instruments
Instruments

NovaSeq X Series: optimized for comprehensive omic solutions

Expand biological discovery with the power of high-throughput multiomics 

Harness omic solutions with the NovaSeq X Series 

The NovaSeq X Series delivers the scalable sequencing power and flexibility to support a broad range of methods for interrogating the genome, epigenome, transcriptome, and proteome. Engineered to output data with the highest integrity, the NovaSeq X Series makes even the most data-intensive research accessible with industry leading speed and throughput.

Unleash discovery power across multiple omes with innovations designed for the NovaSeq X Series:

Flexibility

Dual-flow cell architecture and up to 16 individually addressable lanes support broad omic products and applications. Powerful software portfolio from Illumina streamlines data analysis and interpretation across diverse workflows.  

Scalability

Multiple cycle kits support a wide range of scalable options. The NovaSeq X Plus System and 1.5B, 10B, and 25B flow cells enable data-intensive multiomic studies at a broader and deeper scale than ever before.

Efficiency

Reduced touch points and steps allow faster and easier workflows from sample preparation to data interpretation. Individually addressable lanes help tailor sequencing data per library.

Cost

Process more samples and access more omes with up to 3× higher accuracy.1 The 25B flow cell increases the number of samples by up to 2.5× for the same budget.2

  1. With XLEAP-SBS Chemistry
  2. Compared to the NovaSeq 6000 S4 flow cell.
  • Technical note PDF 1 MB
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The NovaSeq X Series: Achieve single-cell discoveries powered by 25 billion reads

The time is now for multiomics to address complex biological questions. Watch the video to discover how scientists are gaining an advantage in their sample-to-discovery journey through the power of the NovaSeq X Series.

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Technical note spotlight

Perturb-Seq on the NovaSeq X 25B flow cell

Learn how you can achieve high-throughput, genome-scale Perturb-Seq experiments with NovaSeq X 25B flow cells. In this technical note, in collaboration with Single Cell Discoveries, we demonstrate a large-scale CRISPR interference workflow. By leveraging the speed and scale of NovaSeq X and 25B flow cells, you will also get insights on how they are making high quality 1M cell Peturb-Seq experiments accessible and efficient. 

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Integrated multiomic workflows for the NovaSeq X Series

1. Library prep solutions

A broad portfolio of high-throughput Illumina and third-party solutions to interrogate the genome, epigenome, trancriptome, and proteome.

2. Sequencing with NovaSeq X Series

The NovaSeq X and NovaSeq X Plus Sequencing Systems provide exceptional throughput and accuracy for your data-intensive applications at scale.

3. Illumina Connected Analytics

Experience the power of our secure and flexible bioinformatics platform, designed to operationalize informatics workflows and drive groundbreaking scientific insights.

Omic methods and workflows with the NovaSeq X Series

The NovaSeq X Series makes multiomics accessible to enable discoveries that were previously out of reach. Explore products and reagents, with supporting application notes, resources, and relevant analysis solutions to interpret results for omics workflows below. 

By studying the genome, researchers can use information about the structure, function, and sequence of DNA to better understand its role in health and disease.

Featured solutions and resources

Illumina DNA PCR-Free Prep

A rapid and flexible workflow for sensitive whole-genome sequencing.

Illumina DNA Prep

A fast, robust, and flexible workflow for a wide range of DNA input types.

Illumina DNA Prep with Enrichment

The fastest and most flexible targeted DNA sequencing solution from Illumina.

Illumina Cell-Free DNA Prep with Enrichment

A highly sensitive library preparation solution to detect low-abundance mutations from cell-free DNA samples. Learn more in our Data Sheet.

Illumina Complete Long Reads

Explore products for Illumina Complete Long Read sequencing.

The transcriptome includes all RNA molecules in an organism and is an important area of study to understand the function of the genome, which can reveal insights into diseases and drug discoveries.

Featured solutions and resources

Illumina Stranded Total RNA Prep with Ribo-Zero Plus

Get an accurate measurement of gene and transcript abundance and detect both known and novel features in coding and multiple forms of noncoding RNA. Learn more in our Data Sheet.

Illumina RNA Prep with Enrichment

A fast, integrated workflow for producing enriched and indexed sequencing libraries from a broad range of sample types and RNA input quantities. Learn more in our App Note.

Illumina Stranded mRNA Prep

A streamlined solution for clear and comprehensive analysis across the coding transcriptome for discovery of features such as novel isoforms, gene fusions, and allele-specific expression. Learn more in our Data Sheet.

Analysis for single-cell RNA sequencing

Read how the Illumina BaseSpace Sequence Hub now supports ScaleBio’s Single Cell RNA Sequencing Kit, a combinatorial indexing and instrument-free approach to achieve highly multiplexed library preparation on hundreds of thousands of cells for single cell RNA analysis.

Spatial transcriptomics data from Curio Bioscience

High-throughput spatial sequencing platform that enables whole transcriptome analysis of fresh frozen tissue at single-cell scale resolution.

Explore the transcriptome with single‑cell resolution

Measure gene expression across tens of thousands of single cells and analyze cellular heterogeneity.

10x Genomics’ Visium FFPE with CytAssist

Learn how spatial transcriptomics experiments combine molecular and morphological characterization to provide a previously inaccessible view of tissue biology. 

Accelerating single-cell analysis technology for multiomics

Read this Press Release to learn how Illumina plans on developing single-cell technologies to support the latest multiomics solutions.

Insights from gene expression and regulation studies

Download this eBook to learn how to get accurate, versatile, and integrated data analysis solutions for high-impact research with Illumina Connected Software.

Illumina Single-Cell 3' RNA Prep

Learn how Illumina single-cell technology offers an easy, scalable workflow, making scRNA-seq more accessible without the budget or technology limitations of other single-cell methods.

Epigenomics is the complete study of epigenetic changes of many genes within a cell or organism other than DNA sequence changes.

Featured solutions and resources

ZYMO-Seq whole-genome bisulfite sequencing

Read more about whole Genome bisulfite sequencing (WGBS) for DNA methylation studies to quantify base-level methylation.

Unify single-cell gene expression and chromatin accessibility

Connect single-cell epigenome and transcriptome results for a holistic view of gene regulation from the same single cells in this Technical Note.

ATAC-Seq solutions and resources to analyze chromatin accessibility

 Explore products such as the Illumina Tagment DNA TDE1 Enzyme and Buffer Kits to analyze chromatin accessibility in ATAC-Seq experiments. Also, see resources for ATAC-Seq and a webinar on how this method was used in single-cell experiments and more.

Discover the combinatorial power of genetics and epigenetics

Learn more about biomodal’s duet multiomics solution +modC data that now available on Illumina BaseSpace Sequence Hub.

Proteomics is the complete study of the structure and function of proteins in an organism. The development of high-throughput proteomics using NGS can advance biomarker discoveries and improve our understanding of cellular functions and diseases. 

Featured solutions and resources

Combine protein and gene expression profiling

In this Application Note, see how to use methods such as BEN-Seq (bulk epitope and nucleic acid sequencing) and CITE-Seq (cellular indexing of transcriptomes and epitopes sequencing) to analyze protein and gene expression profiling in Th1 cells.

Merge transcriptomic and phenotypic data with CITE-Seq

Learn how CITE-Seq can simultaneously analyze the transcriptome and protein expression at single-cell resolution. In addition, see how researchers use similar methods to reveal new cell types and states associated with disease in this article.

Analyzing CITE-Seq data

Learn how to leverage the DRAGEN Single-Cell RNA app on the Illumina BaseSpace Sequence Hub to analyze multiplexed samples (mRNA and protein).

Illumina Protein Prep

Aptamer-based proteomics solution with NGS readout to identify and quantify 9.5K unique human proteins. Learn more in our data sheet.

Multiplex high-throughput proteomics with exceptional analytical specificity

Read this Technical Note to learn how to leverage Olink Explore and Illumina sequencing systems to perform high-throughput protein profiling for biomarker and drug discovery studies.

Spatial proteogenomics workflows and analysis with Illumina BaseSpace Sequence Hub

See how to combine spatial information with protein and RNA expression using NanoString’s GeoMx DSP platform and Illumina BaseSpace Sequence Hub, a cloud-based genomics hub for NGS data management and analysis. See the related Application Note to see how it can resolve the tissue architecture of astrocytoma and glioblastoma.

Omics data analysis and interpretation solutions

Illumina offers a comprehensive portfolio of software solutions to analyze omic data and lead to novel insights. Explore informatic solutions capable of scaling to production-level systems such as the NovaSeq X Series to streamline data to insight with efficiency and ease.

Software solutions

DRAGEN secondary analysis

Get accurate, comprehensive, and highly efficient bioinformatics with multiple deployment options, applications, and pipelines to meet your research needs.  

BaseSpace Sequence Hub

Integrates with Illumina sequencing systems with an intuitive interface for streamlined run setups and monitoring. Enjoy intuitive push-button secondary analysis and data sharing within a secure cloud ecosystem with expandable storage for growing laboratory needs.

Illumina Connected Analytics

A secure, scalable, bioinformatics platform that enables you to manage, analyze, and interpret large volumes of multiomic data. 

Partek Flow analysis solution

Partek Flow bioinformatics software provides a visual user interface, robust statistical algorithms, information-rich visualizations, and cutting-edge genomic tools that enable researchers of all skill levels to confidently perform data analysis. 

Illumina Correlation Engine

An interactive omics knowledgebase that puts private omics data in biological context with highly curated public data.

Software articles and resources

DRAGEN for single-cell genomic insights

Read this update on the introduction of innovative pipelines for DRAGEN, including single-cell ATAC-seq and single-cell multiomics (RNA-Seq and ATAC-seq).

BaseSpace Sequence Hub with 10x Genomics libraries

Learn how sequencing runs with 10x Genomics libraries can now be directly set up on the Run Planner. The Run Planner tool guides you through the steps needed for preparing your sequencing run, such as specifying sample and index information, read length and analysis options.

Partek bioinformatics for spatial multiomics

Learn how to combine RNA and protein data, statistically assess relationships using correlation between gene and protein molecules, and explore differences between cell types using differential analysis comparisons for biological interpretation.

Video resources

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Illumina Connected Analytics for multiomic cancer applications

Watch this Illumina Connected Analytics workflow demo specific to Cohorts for multiomic cancer analysis.

Application note spotlight

Cancer-specific protein patterns of single cells

Download this Application Note to discover how to simultaneously quantify and spatially map membrane proteins using Molecular Pixelation powered by Illumina NGS. Learn how the ability to measure and visualize 80 surface proteins across 1000 single cells per sample—utilizing the Pixelator pipeline and analysis package—can enhance your understanding of immune cell behavior in cancer.

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Multiomics eBooks

The power of multiomics

See how you can use multiomics to better connect genotype to phenotype and obtain a full cellular readout not found through single omics approaches.

Multiomics sequencing with the NovaSeq X Series

With the NovaSeq X Series, learn how you can perform broader, deeper sequencing and take on omics projects that were previously out of reach.

Gene expression and regulation bioinformatics eBook

This eBook outlines the NGS analysis workflow and provides an overview of Illumina Connected Software solutions available for analyzing gene expression and regulation data.

Single-cell sequencing eBook

See how you can use multiomics to better connect genotype to phenotype and obtain a full cellular readout not found through single omics approaches.

Additional resources

TruSight Oncology ctDNA v2 on the NovaSeq X Series

In this app note, we compare the performance of the NovaSeq X and NovaSeq 6000 using the TruSight Oncology ctDNA v2 CGP assay. Results demonstrate the same high level of performance with significantly reduced run times when using the NovaSeq X Series.

Accelerate omics pattern discovery using Correlation Engine

Read how Illumina’s Correlation Engine provides a curated omics knowledge base of biological interactions that is driven by the vast amount of multiomic public data and publications, along with the built-in comprehensive analytic correlation tools.

FAQs

You can learn more at our multiomics page where you’ll see how to expand your research and discovery power across multiple omes.

The NovaSeq X Series is capable of generating large volumes of valuable data which can be analyzed using Illumina Connected Analytics (ICA) for production-scale informatics. ICA provides tight integration with Illumina hardware and software with customizable workflows and built-in security and compliance features to meet regulated environments. In addition, BaseSpace Sequence Hub offers an intuitive interface that allows you to streamline run setups and monitoring using a secure, cloud-based ecosystem suited for growing laboratories.

A variety of solutions are available. Software solutions such as Illumina Connected Analytics and Correlation Engine offer capabilities to analyze microarray and NGS data. Partnered solutions from Partek also offer the ability to analyze NGS, microarray, and qPCR data.

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