Target 1385 cancer genes for gene expression, variant, and fusion detection studies in many RNA sample types, including blood, bone marrow, and FFPE tissue.
Assay time
Hands-on time
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Reagent and library prep kit bundles available for the MiniSeq System
Order MiniSeq reagents and library prep kits together for additional savings and convenience (Catalog no. 20005611).
The TruSight RNA Pan-Cancer Panel offers in-depth and cost-effective assessment of cancer-related RNA transcripts in limited and degraded samples on a benchtop sequencing system.
Libraries can be prepared from as little as 10 ng of total RNA input (or 20 ng from FFPE samples). The focus on a subset of relevant genes enables RNA-Seq with high sensitivity.
Analysis can be performed using the BaseSpace RNA-Seq Alignment App, which provides gene expression profiles, identifies gene fusions, and reports single nucleotide variants and small indels, offering a comprehensive, intuitive solution to cancer researchers.
Register or sign in to BaseSpace Sequence Hub to access MiSeq System data and MiniSeq System data (view run or view project).
| Assay time | 2.5 days |
|---|---|
| Automation details | Explore available automation methods |
| Cancer type | Pan-Cancer, Hematologic, Solid Tumor |
| Content specifications | Pan-cancer panel with 1,385 targeted genes and 21,043 targeted exonic regions. Includes 507 genes involved in cancer gene fusions. |
| Description | Comprehensive assessment of cancer-related RNA transcripts that enables research on gene fusions, variants, and gene expression changes in multiple cancer types. |
| Hands-on time | 11 hr |
| Input quantity | 10 ng total RNA, 20–100 ng FFPE RNA |
| Instruments | MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System |
| Method | Target Enrichment, Targeted RNA Sequencing, Custom Sequencing |
| Nucleic acid type | RNA |
| Specialized sample types | Low-Input Samples, FFPE Tissue |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Gene fusions, Somatic variants, Novel transcripts, Transcript variants |
Requires purchase of TruSight RNA Pan-Cancer Panel Set A and/or B. Each set includes library preparation reagents and oligos for 48 samples with 12 indexes. Each index is sufficient for 4 individual samples. Both sets may be used together to pool up to 24 samples.
If using the MiniSeq System, reagents and library prep kits can be ordered together in the TruSight Pan-Cancer Set A MiniSeq kit.
The TruSight RNA Pan-Cancer Panel provides comprehensive assessment of cancer-related RNA transcripts and fusion detection in FFPE tissue and other oncology samples for research.
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| MiniSeq System | Samples per run: mid output: 2-3, high output: 8 (based on 3 million reads per sample) |
2 × 75 bp (max recommended) |
| MiSeq System | 8 samples per run with v3 reagents (based on 3 million reads per sample) |
2 × 75 bp (max recommended) |
Pan-cancer analysis with NGS comprehensively assesses gene variants and abnormalities associated with many common cancers, regardless of tumor origin.
Detect cancer gene expression and transcriptome changes and identify novel cancer transcripts with RNA next-generation sequencing (RNA-Seq).
Low-quality/FFPE RNA-Seq solutions
Find FFPE RNA sequencing solutions that yield high-quality results from degraded FFPE tissues and other low-quality samples.
| TruSight RNA Pan-Cancer Panel | TruSight RNA Fusion Panel | |
|---|---|---|
| Assay time | 2.5 days | 2.5 days |
| Automation details | Explore available automation methods | Explore available automation methods |
| Cancer type | Pan-Cancer, Hematologic, Solid Tumor | Pan-Cancer, Hematologic, Solid Tumor |
| Content specifications | Pan-cancer panel with 1,385 targeted genes and 21,043 targeted exonic regions. Includes 507 genes involved in cancer gene fusions. | |
| Description | Comprehensive assessment of cancer-related RNA transcripts that enables research on gene fusions, variants, and gene expression changes in multiple cancer types. |
Provides a cost-effective NGS solution for cancer research, offering deep coverage of 507 genes implicated in multiple cancer types, including solid tumors, sarcomas, and hematological malignancies. |
| Hands-on time | 11 hr | 11 hr |
| Input quantity | 10 ng total RNA, 20–100 ng FFPE RNA | 10 ng total RNA, 20–100 ng FFPE RNA |
| Instruments | MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System | MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System |
| Method | Target Enrichment, Targeted RNA Sequencing, Custom Sequencing | Target Enrichment, Targeted RNA Sequencing |
| Nucleic acid type | RNA | RNA |
| Specialized sample types | Low-Input Samples, FFPE Tissue | Low-Input Samples, FFPE Tissue |
| Species category | Human | Human |
| Technology | Sequencing | Sequencing |
| Variant class | Gene fusions, Somatic variants, Novel transcripts, Transcript variants | Gene fusions |
Library Prep and Array Kit Selector
Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits.
TruSight Pan-Cancer capture chemistry
The TruSight RNA PanCancer Panel provides a simple, streamlined method for isolating targeted regions of interest from total RNA.
Identification of novel gene fusion partners
(A) The size distribution of total RNA from 3 different FFPE samples derived from melanoma are shown on a Bioanalyzer. (B) Table shows the detection of the gene fusion partner in FFPE samples when only one gene (highlighted) of the fusion pair is targeted.
TruSight RNA Pan-Cancer Panel Set A
RS-303-1002
Includes library preparation reagents and oligos for 48 samples with 12 indexes; each index is sufficient for 4 individual samples. May be used with TruSight RNA Pan-Cancer Panel Set B to allow for pooling up to 24 samples.
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TruSight RNA Pan-Cancer Panel Set B
RS-303-1003
Includes library preparation reagents and oligos for 48 samples with 12 indexes; each index is sufficient for 4 individual samples. May be used with TruSight RNA Pan-Cancer Panel Set A to allow for pooling up to 24 samples.
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TruSight RNA Pan-Cancer Set A MiniSeq Kit
20005611
Includes library preparation reagents and oligos for 48 samples with 12 indexes, plus 6 MiniSeq High Output Kits (150 Cycles).
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Trusight RNA Pan-Cancer Oligo Panel
20046104
Trusight RNA Pan-Cancer Oligo Panel includes oligos sufficient for 96 total RNA samples for sequencing if using 1-plex enrichment or 288 total RNA samples for sequencing if using 3-plex* enrichment. No library prep is included with this panel. *Only applicable to library prep workflows that permit multiplexed enrichment.
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The TruSight RNA Pan-Cancer workflow involves six major steps:
The probes are designed to target 1385 cancer genes and detect fusions by spanning all exons of every gene.
Run the RNA-Seq workflow (FASTQ only) on the MiSeq System and stream the data to BaseSpace Sequence Hub. The BaseSpace RNA-Seq Alignment App analyzes data from the TruSight RNA Pan-Cancer Panel, providing a simple results summary that includes a fusion table, variant table, and gene expression table. You may also use your own pipeline for analysis.
Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
Offers a 90-min workflow to prepare DNA libraries for small genomes, PCR amplicons, plasmids, and cDNA sequencing, with a low DNA input requirement.
Reagent v2.5 kits use a resilient flow cell that maintains starting fluorescent intensity, improving stability and performance compared to earlier versions.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Targeted DNA and RNA research panel investigating variants across 161 genes associated with a range of cancer types.
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